| 高效液相色谱法测定人血浆中盐酸氨溴索的浓度 |
| 投稿时间：2011-10-26 修订日期：2011-12-25 点此下载全文 |
| 引用本文：宋晓楠,王晓波,袭荣刚,马文辉,闫冬.高效液相色谱法测定人血浆中盐酸氨溴索的浓度[J].药学实践杂志,2012,30(1):29~31 |
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| 中文摘要:目的 建立HPLC测定人血浆中盐酸氨溴索含量的方法。 方法 选用Diamonsil-C18柱(200 mm×4.6 mm,5μm),流动相:甲醇-0.01 mol/L磷酸盐缓冲液(pH 7.0)-四氢呋喃(700:320:25,v/v/v),流速:1.2 ml/min,检测波长:242 nm,柱温:室温。 结果 该方法低、中、高3种浓度的提取回收率为88.2％ ~104.8％ ,日内、日间相对标准差为2.7％~5.3％(n=6),在10.2~610.8 ng/ml范 |
| 中文关键词:盐酸氨溴索 高效液相色谱 血药浓度 |
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| Determination of ambroxol hydrochloride in humam plasma by HPLC |
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| Abstract:Objective To develop a fast reverse-phase high-performance liquid chromatography assay method for the determination of ambroxol hydrochloride. Methods A Diamonsil-C18(200 mm×4.6 mm,5μm)column was used.The mobile phase consisted of methanol-0.01 mol/L PBS(pH 7.0)-THF(700:320:25,v/v/v).Detection wavelength was 242 nm. Results A good linear relationship was obtained in the concentration range of 10.2~610.8 ng/ml. The average recovery of this method was 88.2％~104.8％. The RSD of within-day and between-day were all less than 15％. Conclusion The method was simple,rapid and accurate. The result showed that the method was suitable for the determination of ambroxol hydrochloride. |
| keywords:Ambroxol hydrochloride HPLC Plasma concentration |
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