| RP-HPLC法测定中药木贼中黄酮醇苷的含量 |
| 投稿时间：2010-07-13 修订日期：2009-08-31 点此下载全文 |
| 引用本文：李颜,朱金辉,李丹,郭澄.RP-HPLC法测定中药木贼中黄酮醇苷的含量[J].药学实践杂志,2011,29(1):33~34,37 |
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| 中文摘要:目的 建立以高效液相法测定中药木贼中一个黄酮醇苷山奈酚-3-双葡萄糖苷的含量方法。 方法 色谱柱为Calesil ODS-100(250 mm×4.6 mm, 5 μm),流动相为甲醇-水(45:55),流速为1.0 ml/min, 检测波长254 nm, 柱温30 ℃,进样量10 μl。 结果 山奈酚-3,7-双葡萄糖苷的检测浓度在0.003 12~0.104 mg/ml范围内与峰积分面积呈良好线性关系(r=0.999 9), 平均回收率为100.2%,RSD=1.74% (n=9)。3个不同产地批号的样品中山奈酚-3-双葡萄糖苷的含量均在0.30%以上。 结论 该方法简便、快捷、重复性好,可作为木贼药材的质量控制的方法。 |
| 中文关键词:木贼 黄酮醇苷 山奈酚-3-双葡萄糖苷 反相高效液相法 含量测定 |
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| Determination of a flavonol glycoside in Equisetum hiemale by RP-HPLC |
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| Abstract:Objective To establish a RP-HPLC method for determination of a flavonol glycoside in Equisetum hiemale. Methods The separation was performed on Calesil ODS-100(250 mm×4.6 mm,5 μm). Mobile phase consisted of methanol-water (45:55) with flow rate of 1.0 ml/min and detection wavelength of 254 nm. The column temperature was 30 ℃ and injection volume was 10 μl. Results The linear range of the flavonol glycoside was 0.003 12~0.104 mg/ml with average recovery of 100.2% ( RSD=1.74%, n=9 ). The method was applied to samples of Equisetum hiemale from different locations. Conclusion The established method was proved to be simple, reproducible, accurate and could be used to evaluate the quality of Equisetum hiemale. |
| keywords:Equisetum hiemale flavonal glycoside kaempferol-3-diglucoside RP-HPLC Content determination |
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