| 7种美白化合物对皮肤黑素细胞的体外生物学效应 |
| 投稿时间：2010-07-05 修订日期：2010-12-17 点此下载全文 |
| 引用本文：张玮,高静,张敏,王晓宇,侯雪梅,高申.7种美白化合物对皮肤黑素细胞的体外生物学效应[J].药学实践杂志,2011,29(1):24~26,40 |
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| 基金项目:国家自然基金:项目编号:NO.81000689;"重大新药创制"科技重大专项(2008ZXJ09010-001);上海市自然基金:项目编号10ZR1437300. |
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| 中文摘要:目的 研究7种美白化合物对体外培养的皮肤黑素细胞的生物学效应。 方法 利用B-16小鼠黑素瘤细胞株,以左旋多巴为底物测定细胞内酪氨酸酶活性,采用比色法测定细胞中黑色素含量,以溴化二苯四偶氮法(MTT法)测定化合物对黑素细胞活力的影响。 结果 各化合物对黑素细胞酪氨酸酶活性和黑素生成量呈浓度依赖性抑制,对黑素细胞活力均有不同程度的影响。 结论 这7种化合物均能抑制黑素细胞的黑色素生成。 |
| 中文关键词:美白化学物 黑素细胞 黑色素 酪氨酸酶 |
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| Bio-efficacy of seven skin-lighteners on dermal melanocytes in vitro |
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| Abstract:Objective To investigate the bio-efficacy of seven skin-lighteners on melanocytes in vitro. Methods After B-16 melanoma cells were treated with skin-lightener, the tyrosinase activity, the melanin content and cell viability were measured by utilization of L-Dopa as the substrate, colorimetric analysis, and MTT assay, respectively. Results each of the skin-lighteners showed a concentration-dependent reduction in tyrosinase activity and the melanin content and partially inhibited the cell viability. Conclusion all of the seven skin-lighteners could inhibit the melanocytes to produce melanin. |
| keywords:skin-lightener melanocyte melanin tyrosinase |
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