| 二至丸水提物对体外肝细胞损伤的保护作用 |
| 投稿时间：2010-04-30 修订日期：2010-05-27 点此下载全文 |
| 引用本文：闫冰,丁安伟,张丽.二至丸水提物对体外肝细胞损伤的保护作用[J].药学实践杂志,2010,28(6):433~439,436 |
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| 基金项目:江苏省中医药管理局研究项目(LB09031) |
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| 中文摘要:目的:研究二至丸水提物(aqueous extract of Erzhi Pill,AEEP)对体外肝细胞损伤的保护作用及其机制。方法:培养L-O2型肝细胞,采用H2O2和CCl4体外分别诱导肝细胞损伤,检测培养上清液中天门冬氨酸转换酶(AST)和丙氨酸氨基转换酶(ALT)水平,测定上清液中丙二醛(MDA)的含量和过氧化物岐化酶(SOD)活力,MTT法检测细胞存活和增殖活性。结果:①AEEP(0.32-40μg/ml)剂量组可明显降低由H2O2升高的肝细胞培养上清液中AST和ALT水平及MDA含量,还可提高H2O2降低的肝细胞存活率和SOD活力;②AEEP(0.3240μg/ml)剂量组可使CCl4升高的肝细胞培养上清液中ALT和ALT水平及MDA含量明显降低或恢复,还可提高CCl4降低的肝细胞存活率和SOD活力。结论:提示AEEP对体外肝细胞损伤有直接保护作用,该作用可能与其抗氧化作用有关。 |
| 中文关键词:二至丸 L-O2型肝细胞 四氯化碳(CCl4) 过氧化氢(H2O2) 抗氧化作用 |
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| Protective effects of aqueous extract of Erzhi pill on hepatocytes injured by CCl4 or H2O2 |
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| Abstract:Objective To study the protective effects and mechanism of aqueous extract of Erzhi pill on hepatocytes injured by CCL4 or H2O2.Methods The L-O2 hepatocytes were incubated and then injured by CCl4.The levels of AST,ALT,MDA and SOD in cultural supernatant were detected by general Methods.Cell viability was assayed by MTT method.Results The elevation of MDA content of hepatocytes and AST,ALT level in supernatant of hepatocytes,and the loss of SOD activit y induced by CCl4 were restored remarkably by AEEP(0.32～40 μg/ml).The elevation of ALT,AST level in the supernatant of hepatocytes and MDA content of hepatocytes,and the loss of SOD activity induced by H2O2 were improved significantly by AEEP(0.32～40 μg/ml)treatment.Conclusion The Results suggest that AEEP possess direct protective action on hepatocytes in vitro injured by CCl4 or H2O2.These might be associated with its anti-oxidative activity. |
| keywords:Erzhi Pill L-O2 hepatocytes CCl4 H2O2 anti-oxidative activity |
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