| 短指软珊瑚中的生态活性物质 |
| 投稿时间：2010-06-21 修订日期：2010-07-16 点此下载全文 |
| 引用本文：孟丽媛,李秀保,张文,李玲,孙鹏,汤华,刘宝姝,易杨华.短指软珊瑚中的生态活性物质[J].药学实践杂志,2010,28(4):274~278 |
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| 基金项目:国家自然基金项目(30873200);上海市基金项目(08JC1405600);上海市浦江人才计划项目(PJ2008);上海市重点学科建设项目(B906) |
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| 中文摘要:目的:研究南海短指软珊瑚(Sinularia sp.)的生态活性物质。方法:利用硅胶柱色谱、Sephadex LH-20及HPLC等层析手段,对短指软珊瑚粗提物进行分离纯化,应用现代波谱技术(NMR、MS等)鉴定化合物结构。结果:从该珊瑚中分离鉴定了7个甾醇类化合物,分别是:麦角甾-7,24(28)-二烯-3β,5α,6β-三醇(1),(24R)麦角甾7,22二烯-3β,5α,6β-三醇(2),麦角甾-24(28)-烯-3β,5α,6β-三醇(3),(24S)-24-甲基-麦角甾-7-烯-3β,5α,6β三醇(4),23-去甲基柳珊瑚甾-7-烯-3β,5α,6β-三醇(5),过氧化麦角甾醇(6),胆甾醇(7)。结论:除海洋无脊椎动物中常见的胆甾醇和过氧化麦角甾醇外,这些化合物均为具有3β,5α,6β-三羟基结构片段的麦角甾醇类似物。初步的体外活性测试实验表明这些化合物对细菌、真菌和藻类有不同程度的抑制作用。 |
| 中文关键词:短指软珊瑚 多羟基甾醇 抗微生物活性物质 |
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| Bioactive constituents from Sinularia sp |
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| Abstract:ObjectiveTo study the bioactive constituents of soft coral Sinularia sp.Methods The compounds were isolated and purified by silica gel,Sephadex LH-20 column chromatography and HPLC.Their chemical structures were identified by spectroscopic analysis.Results Seven sterol compounds were identified that are: ergos-7,24(28)-dien-3β,5α,6β-triol(1),(24R)-ergost-7,22-dien-3β,5α,6β-triol(2),ergost-24(28)-en-3β,5α,6β-triol(3),(24 S)-ergost-7(8)-en-3β,5α,6β-triol(4),23-demethylgorgost-7-en-3β,5α,6β-triol(5),ergosterol peroxide(6),cholesterol(7).Conclusions All these compounds have the ergost-3β,5α,6β-tiol substructure segment except the common ergosterol peroxide and cholesterol.Preliminary bioactive experiment indicated that these compounds have the effect of antimicrobial activity. |
| keywords:Sinularia sp. polyhydroxyled sterol antimicrobial constituents |
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