| 正极性驻极体联合5-氟尿嘧啶对瘢痕成纤维细胞生长抑制的协同作用 |
| 投稿时间：2023-10-16 修订日期：2024-03-19 点此下载全文 |
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| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要:目的 探讨驻极体及药物5-氟尿嘧啶（5-FU）对瘢痕成纤维细胞生长和迁移的影响及其可能的作用机制。方法 检测不同表面电位的正极性驻极体联合5-FU对瘢痕成纤维细胞的影响。采用R-T PCR技术研究了在静电场下生长的成纤维细胞中p53等人mRNA的表达变化。结果 ①正极性驻极体和不同浓度5-FU联用作用于细胞72h后，细胞增殖率都有所降低，对瘢痕细胞的抑制率到达0.15±0.051。②+5000V 驻极体组可促进瘢痕成纤维细胞凋亡； +5000V 驻极体与 5-FU联用组细胞凋亡数高于 5-FU 单一使用组。③ +5000V 驻极体作用组，四种凋亡基因 mRNA 的相对表达量都有所增加， +5000V 驻极体与 5-FU 联合作用组四种标志性基因表达量均较 5-FU 组增大。结论 正极性驻极体与5-FU联合作用对抑制细胞生长有协同作用。正极性驻极体抑制瘢痕细胞生长的机制可能是通过促进细胞凋亡基因的表达，进而影响细胞的生长状态来抑制细胞生长。 |
| 中文关键词:静电场，驻极体，5-氟尿嘧啶，瘢痕成纤维细胞 |
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| Synergistic effect of positive electret combined with 5- fluorouracil on growth inhibition of scar fibroblasts |
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| Abstract:Objective To investigate the effects of electret and 5-fluorouracil (5-FU) on the growth and migration of scar fibroblasts and its possible mechanism. Methods The effects of positive electret with different surface potentials combined with 5-FU on scar fibroblasts were examined. The expression of human mRNA such as p53 in fibroblasts grown under electrostatic field was studied by R-T PCR. Results ① After the treatment of positive electret and different concentrations of 5-FU for 72h, the cell proliferation rate decreased, and the inhibition rate of scar cells reached 0.15±0.051.②+5000V electret group can promote the apoptosis of scar fibroblasts; The number of apoptotic cells in +5000V electret and 5-FU group was higher than that in 5-FU group. ③The mRNA expression levels of four apoptotic genes in the +5000V electret group were increased, and the expression levels of four signature genes in the +5000V electret and 5-FU group were increased compared with those in the 5-FU group. Conclusion The combination of positive electret and 5-FU has a synergistic effect on inhibiting cell growth. The mechanism of positive electret inhibiting scar cell growth may be through promoting the expression of apoptosis gene, and then affecting the growth state of cells to inhibit cell growth. |
| keywords:electrostatic field, electret, 5-fluorouracil, scar fibroblasts |
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