夏枯草消瘤合剂对Lewis肺癌小鼠的药效作用
投稿时间:2018-12-24  修订日期:2019-09-22  点此下载全文
引用本文:马明华,汪晓河,戴媛媛,顾小燕,吴铁军,年华.夏枯草消瘤合剂对Lewis肺癌小鼠的药效作用[J].药学实践杂志,2020,38(1):57~62
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作者单位E-mail
马明华 上海市杨浦区中心医院药剂科, 上海 200090  
汪晓河 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437  
戴媛媛 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437  
顾小燕 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437  
吴铁军 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437  
年华 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437 jackynian@126.com 
基金项目:上海中医药大学高峰高原学科2018年项目(30304114334);中药炮制传承基地项目(30304118220);上海市科学技术委员会科研计划项目(16401902100);上海中医药大学预算内项目(18LK047)
中文摘要:目的 探讨夏枯草消瘤合剂对Lewis肺癌小鼠的治疗作用。方法 采用C57BL/6小鼠Lewis肺癌移植瘤模型,将30只小鼠随机分为3组:模型对照组、夏枯草消瘤合剂组(M组)、顺铂组(DDP组),连续用药14 d。通过对Lewis肺癌小鼠的大体观察,测定肿瘤大小,HE染色方法测定肿瘤病理组织学变化,免疫组化方法测定肿瘤组织的细胞周期蛋白D1(CyclinD1)和P16的表达情况。结果 模型对照组瘤体质量最重,与其他组比较差异均有统计学意义(P<0.05);M组可在一定程度上改善小鼠的生存状态及生活质量;各组小鼠的肿瘤生长曲线及HE染色结果显示:M组可抑制肿瘤细胞的生长,且对正常细胞有保护作用;M组、DDP组均可显著降低CyclinD1阳性表达(P<0.01),但是M组对于提高P16阳性表达的效果不显著。结论 夏枯草消瘤合剂具有较好的抑制肺癌生长的作用。
中文关键词:中医药  夏枯草消瘤合剂  Lewis肺癌  CyclinD1  P16
 
Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice
Abstract:Objective To investigate the therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice.Methods 30 mice with C57BL/6 mouse Lewis lung cancer xenograft model were randomly divided into three groups:model control group, Xiakucao Xiaoliu mixture group (M group), cisplatin group (DDP group). M group and DDP group were administered continuously for 14 days. Through the general observation of Lewis lung cancer mice, tumor size was determined, HE staining method was used to determine the histopathological changes of tumors, and the expression of CyclinD1 and P16 in tumor tissues was determined by immunohistochemistry.Results The tumor weight of the model control group was the heaviest, and the difference was statistically significant compared with other groups. (P<0.05). Survival state and quality of life of mice had been improved to some extent in M group. The results of tumor growth curve and HE staining in each group of mice showed that the growth of tumor cells had been inhibited and normal cells had been protected. The positive expression of CyclinD1 was significantly decreased in M group and DDP group (P<0.01), but the effect of M group on the improvement of P16 positive expression was not significant.Conclusion Xiakucao Xiaoliu mixture had a good effect on inhibiting lung tumor growth.
keywords:Chinese Medicine  Xiakucao Xiaoliu mixture  Lewis lung cancer  CyclinD1  P16
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