| HPLC法同时测定盐酸阿霉素与氯尼达明的含量 |
| 投稿时间：2023-06-27 修订日期：2023-12-07 点此下载全文 |
| 引用本文：孙雨菡,许子艺,廖峻,张翮,樊莉,鲁莹.HPLC法同时测定盐酸阿霉素与氯尼达明的含量[J].药学实践杂志,2024,42(3):127~130 |
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| 中文摘要:目的 建立同时测定盐酸阿霉素（DOX·HCl）与氯尼达明（LND）含量的测定方法。方法 采用HPLC法，色谱柱为Agilent 5 HC-C18（2）（4.6 mm×250 mm ，5 μm），流动相为甲醇-0.1%TFA水溶液，梯度洗脱，甲醇比例随时间变化为：0~3 min， 65%甲醇；3~7 min， 65%→90%甲醇；7~13 min，90%甲醇；13~15 min，90%→65%甲醇；15~20 min、65%甲醇。采集时间20 min，平衡时间3 min，紫外检测波长205 nm及253 nm，流速1.0 ml/min，柱温35 ℃，进样量：10 μl。结果 该方法专属性好，盐酸阿霉素在1~40 μg/ml 的浓度范围内线性良好，氯尼达明在6~240 μg/ml的浓度范围内线性良好。该方法之下，两种化合物的精密度、稳定性、回收率均符合要求。结论 建立了同时检测盐酸阿霉素与氯尼达明含量的液相分析方法，该方法专属性强，准确可靠。 |
| 中文关键词:氯尼达明 盐酸阿霉素 高效液相色谱法 联合用药 梯度洗脱 |
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| Content measurement of doxorubicin hydrochloride and lonidamine by HPLC |
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| Abstract:Objective To establish a method for the simultaneous determination of DOX·HCl and LND. Methods HPLC was performed on Agilent 5 HC-C18（2） （4.6 mm×250 mm, 5 μm） column. The mobile phase was methanol-0.1% TFA aqueous solution, and the gradient elution procedure were: 0 to 3 min, 65% methanol; 3 to 7 min, 65%→90% methanol; 7 to 13 min, 90% methanol; 13 to 15 min, 90%→65% methanol; 15 to 20 min, 65% methanol. The collection time was 20 min, the balance time was 3 min, the UV detection wavelengths were 205 nm and 253 nm. The flow rate was 1.0 ml/min and the column temperature was 35℃. The amount of inlet was 10 μl. Results The method was highly specific, and both DOX·HCl and LND exhibited good linearity in the concentration range of 1-40 μg/ml and 6-240 μg/ml, respectively. The two compounds’ precision, stability, and recovery satisfied the requirements of the method. Conclusion This study established a HPLC method that was suitable for the simultaneous detection of DOX·HCl and LND. This method’s high level of specificity, accuracy, and reliability . |
| keywords:lonidamine doxorubicin hydrochloride HPLC drug combination gradient elution |
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