| 实脾消水凝胶贴膏的薄层色谱鉴别和含量测定研究 |
| 投稿时间：2022-05-16 修订日期：2023-05-16 点此下载全文 |
| 引用本文：马秉智,梁莹莹,王海洋,唐永和,李栋,赫军.实脾消水凝胶贴膏的薄层色谱鉴别和含量测定研究[J].药学实践杂志,2023,41(11):662~665,679 |
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| 基金项目:中日友好医院2018年院级科研基金资助项目（编号：2017-2-QN-14）；2023年全国中药特色技术传承人才培训项目（编号：T20234832005）；中日友好医院“菁英计划”人才培养工程（No.ZRJY2021-TD06） |
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| 中文摘要:目的 建立实脾消水凝胶贴膏的薄层色谱鉴别和含量测定方法。方法 采用薄层色谱法（TLC）对制剂中的黄芪、车前子、莪术、桂枝、猪苓、预知子进行鉴别；采用超高效液相色谱‐串联质谱法（UPLC-MS）对制剂中君药黄芪所含成分黄芪甲苷进行含量测定。结果 TLC鉴别图斑点清晰，分离度好，阴性对照无干扰；黄芪甲苷的质量浓度在2.75～33 μg/ml范围内与峰面积积分值呈良好的线性关系(r=0.999 9，n=6)，精密度、重复性、稳定性实验的RSD<3%，平均加样回收率为100.49%（RSD=1.98%，n=6）。结论 本研究所建立的方法准确可靠、专属性强，可用于实脾消水凝胶贴膏的质量控制。 |
| 中文关键词:实脾消水凝胶贴膏 质量标准 薄层色谱法 超高效液相色谱‐串联质谱法 黄芪甲苷 |
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| TLC identification and quantitative determination of Shipi Xiaoshui gel plaster |
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| Abstract:Objective To establish the method of thin layer chromatography (TLC) for identification and quantitative determination of Shipi Xiaoshui gel plaster. Methods TLC was adopted to qualitatively identify astragalus radix, plantaginis semen, curcumae rhizome, cinnamomi ramulus, polyporus umbellatus and akebia quinata. UPLC-MS was used to determine the content of astragaloside Ⅳ. Results TLC spots were clear and well-separated; RSDs of precision, reproducibility and stability tests were all lower than 3%, the linear range of astragaloside Ⅳ was 2.75-33 μg/ml (r=0.999 9, n=6), and the average recovery was 100.49% (RSD=1.98%, n=6). Conclusion The established method in this study is accurate, reliable and specific, which couldbe used for the quality control of Shipi Xiaoshui gel plaster. |
| keywords:Shipi Xiaoshui gel plaster quality standard thin layer chromatography UPLC-MS astragaloside Ⅳ |
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