正极性驻极体联合5-氟尿嘧啶对瘢痕成纤维细胞生长抑制的协同作用
投稿时间:2023-10-16  修订日期:2024-03-19  点此下载全文
引用本文:杨媛媛,安晓强,许佳捷,江键,梁媛媛.正极性驻极体联合5-氟尿嘧啶对瘢痕成纤维细胞生长抑制的协同作用[J].药学实践杂志,2024,42(6):244~247
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作者单位E-mail
杨媛媛 海军军医大学卫生勤务学系, 上海 200433  
安晓强 海军军医大学卫生勤务学系, 上海 200433  
许佳捷 海军军医大学卫生勤务学系, 上海 200433  
江键 海军军医大学卫生勤务学系, 上海 200433 jjiang@smmu.edu.cn 
梁媛媛 海军军医大学卫生勤务学系, 上海 200433 liangyy_208@163.com 
基金项目:国家自然科学基金(51477175)
中文摘要:目的 探讨驻极体及5-氟尿嘧啶(5-FU)对瘢痕成纤维细胞生长的影响及其可能的作用机制。方法 利用全自动酶标仪检测+5000 V驻极体联合不同浓度5-FU对瘢痕成纤维细胞增殖的影响,利用荧光显微镜及RT- PCR技术研究在静电场作用下的瘢痕成纤维细胞的凋亡及p53等凋亡基因mRNA的表达变化。结果 ①正极性驻极体和不同浓度5-FU联用作用于细胞72 h后,细胞增殖率都有所降低,+5000 V驻极体+160 μg/ml 5-FU组对瘢痕细胞的抑制率达到(0.15±0.051)%。② +5000 V 驻极体组可促进瘢痕成纤维细胞凋亡; +5000 V 驻极体与 5-FU联用组细胞凋亡数高于 5-FU 单一使用组。③ +5000 V 驻极体作用组,4种凋亡基因 mRNA 的相对表达量都有所增加, +5000 V 驻极体与 5-FU 联合作用组4种标志性基因表达量均较 5-FU 组增大。结论 正极性驻极体与5-FU联合作用对抑制细胞生长有协同作用。正极性驻极体抑制瘢痕细胞生长的机制可能是通过促进细胞凋亡基因的表达,进而影响细胞的生长状态来抑制细胞生长。
中文关键词:静电场  驻极体  5-氟尿嘧啶  瘢痕成纤维细胞
 
Synergistic effect of positive electret combined with 5-fluorouracil on growth inhibition of scar fibroblasts
Abstract:Objective To investigate the effects and possible mechanism of electret and 5-fluorouracil(5-FU)on the growth of scar fibroblasts. Methods The effect of +5000 V electret combined with different concentrations of 5-FU on the proliferation of scar fibroblasts was detected by automatic enzyme labeling instrument. The apoptosis of scar fibroblasts and the mRNA expression of p53 and other apoptotic genes were studied by fluorescence microscopy and RT-PCR technology under the action of electrostatic field. Results ① After the treatment of positive electret and different concentrations of 5-FU for 72 h, the cell proliferation rate decreased, and the inhibition rate of scar cells in the +5000 V electret+160 μg/ml 5-FU group was (0.15±0.051)%. ②+5000 V electret group could promote the apoptosis of scar fibroblasts; The number of apoptotic cells in +5000 V electret and 5-FU group was higher than that in 5-FU group. ③The mRNA expression levels of four apoptotic genes in the +5000 V electret group were increased, and the expression levels of four signature genes in the +5000 V electret and 5-FU group were increased compared with those in the 5-FU group. Conclusion The combination of positive electret and 5-FU had a synergistic effect on inhibiting cell growth. The mechanism of positive electret inhibiting scar cell growth may be through promoting the expression of apoptosis gene, and then affecting the growth state of cells to inhibit cell growth.
keywords:electrostatic field  electret  5-fluorouracil  scar fibroblasts
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