| 巨噬细胞膜杂合脂质体的制备及抗创伤弧菌溶血素A的作用研究 |
| 投稿时间：2022-07-01 修订日期：2022-10-09 点此下载全文 |
| 引用本文：代宇,郭灵怡,王宏播,卞康晴,俞媛.巨噬细胞膜杂合脂质体的制备及抗创伤弧菌溶血素A的作用研究[J].药学实践杂志,2023,41(1):26~30,55 |
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| 基金项目:国家自然科学基金 (82273487)；全军医学科技青年培育计划（21QNPY051） |
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| 中文摘要:目的 以巨噬细胞膜和脂质体杂合，制备仿生纳米载体-巨噬细胞膜杂合脂质体，用于创伤弧菌溶血素A（VvhA）的清除及毒性抑制。方法 提取巨噬细胞膜，通过薄膜挥发-挤出法与脂质体杂合，构建巨噬细胞膜杂合脂质体并进行表征；通过溶血实验和细胞毒性实验对杂合载体的体外解毒能力进行评价，小鼠皮肤感染模型来评价载体的解毒能力。结果 体内外抗类毒素研究表明，巨噬细胞膜杂合脂质体的体外抗溶血率达到97.03%，能够有效抑制皮下感染小鼠的皮肤溃烂，并使腹腔感染小鼠的生存率达到80%。结论 构建的巨噬细胞膜杂合脂质体对创伤弧菌外毒素VvhA引起的细胞及皮肤组织毒性具有良好的抑制作用。 |
| 中文关键词:创伤弧菌 溶血素A 仿生纳米载体 细菌感染 |
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| Preparation and anti-Vibrio vulnificus hemolysin A of macrophage membrane hybrid liposome |
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| Abstract:Objective To prepare a biomimetic nano carrier macrophage membrane hybrid liposome by heterozygous macrophage membrane and liposome, which could be used for the clearance and toxicity inhibition of Vibrio vulnificus hemolysin A (VvhA). Methods Macrophage membrane was extracted and hybridized with liposome by thin-film evaporation combined extrusion method. The hybridized liposome of macrophage membrane was constructed and characterized. The in vitro detoxification ability of the hybridized vector was evaluated by hemolysis test and cytotoxicity test. The detoxification ability of the vector was evaluated by mouse skin infection model. Results Anti toxoid studies in vivo and in vitro showed that the anti-hemolysis rate of macrophage membrane heterozygous liposomes in vitro reached 97.03%, which could effectively inhibit the skin ulceration in subcutaneous infected mice and make the survival rate of abdominal infected mice reach 80%. Conclusion The constructed macrophage membrane hybrid liposome had high detoxification ability, which could provide a potential solution and research basis for the prevention and treatment of Vibrio vulnificus infection. |
| keywords:Vibrio vulnificus hemolysin A biomimetic nanocarrier bacterial infections |
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