| UHPLC法测定辐射后对乙酰氨基酚缓释片含量 |
| 投稿时间：2021-09-18 修订日期：2021-11-24 点此下载全文 |
| 引用本文：于德勋,黄心慧,朱可涵,黄婷,周婷婷,高建义.UHPLC法测定辐射后对乙酰氨基酚缓释片含量[J].药学实践杂志,2022,40(6):550~552,562 |
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| 基金项目:军队后勤科研项目（BWS17J028） |
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| 中文摘要:目的 采用超高效液相色谱法,对γ射线辐射的对乙酰氨基酚缓释片进行含量测定。方法 采用60Co对对乙酰氨基酚缓释片进行辐射，使用超高效液相色谱法测定其含量，色谱柱为Shim-pack GISS C18（2.1 mm×50 mm，1.9 μm），以0.05%醋酸铵溶液-甲醇(85∶15)为流动相,流速为0.3 ml/min,检测波长为245 nm。结果 对乙酰氨基酚含量在浓度为20～100 μg/ml范围内线性良好,相关系数r=0.9994，重复性为0.9％，加样回收率97.9 %～104.9 %，在0、8、50和80 kGy辐射量条件下，对乙酰氨基酚含量分别为96.2 %、92.2 %、91.8 %和83.9 %。结论 超高效液相色谱法的分离效果快速、结果准确,适用于经辐射的对乙酰氨基酚缓释片的含量测定，辐射后对乙酰氨基酚含量有所降低。 |
| 中文关键词:对乙酰氨基酚 超高效液相色谱法 含量 辐射 |
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| Acetaminophen content assay with UHPLC in the sustained-release tablet exposed to radiation |
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| Abstract:Objective To assay the contents of acetaminophen with ultra high-performance liquid chromatography (UHPLC) method in the sustained-release tablets radiated by Gamma ray. Methods Acetaminophen sustained-release tablets were radiated by 60Co. UHPLC equipped with the Shim-pack GISS-C18（2.1 mm×50 mm, 1.9 μm）was used for the assay. The mobile phase was methanol-0.05 % ammonium acetate solution (15∶85). The flow rate was 0.3 ml/min with the detection wavelength at 245 nm. Results The acetaminophen showed good linear relationship within the range of 20-100 μg/ml (r=0.999 4). The RSD values of repeatability was 0.9 %. The average recovery was 97.9 %-104.9 %. Acetaminophen content was 96.2 %, 92.2 %, 91.8 %, 83.9 % at 0, 8, 50 and 80 kGy radiation, respectively. Conclusion This method is speedy and accurate. It can be used to assay the content of acetaminophen in the sustained-release tablets after radiation. The content of acetaminophen decreased after radiation. |
| keywords:acetaminophen UHPLC content radiation |
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