| 河豚毒素对斑马鱼的急性毒性研究 |
| 投稿时间：2022-02-28 修订日期：2022-06-22 点此下载全文 |
| 引用本文：王浩能,张吉芊竹,李劲锋,贾睿,朱玉平,朱江波,陈基快,严朗.河豚毒素对斑马鱼的急性毒性研究[J].药学实践杂志,2022,40(6):536~539 |
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| 基金项目:国家重点研发计划项目（2019YFC0312604） |
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| 中文摘要:目的 研究河豚毒素对斑马鱼的急性毒性。方法 在斑马鱼中用最大非致死浓度（MNLC）和10%致死浓度（LC10）测定和评估河豚毒素的急性毒性。结果 经Origin 8.0软件模拟，得出河豚毒素对斑马鱼急性毒性MNLC为8.62 μmol/L，LC10为15.2 μmol/L。在本实验条件下，16.0 μmol/L及以上浓度河豚毒素可诱发斑马鱼心包水肿和心律异常，终点时出现部分或全部死亡。河豚毒素的急性毒性靶器官是心脏和肝脏，主要表现为心包水肿、心律异常和卵黄囊吸收延迟，毒性出现浓度为0.958 μmol/L。结论 河豚毒素对斑马鱼具有一定的心脏和肝脏毒性，且其毒性与河豚毒素的浓度相关。 |
| 中文关键词:河豚毒素 斑马鱼 急性毒性 |
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| The acute toxicity of tetrodotoxin to zebra fish |
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| Abstract:Objective To study the acute toxicity of tetrodotoxin to zebra fish. Methods The maximum non-lethal concentration (MNLC) and 10% lethal concentration (LC10) determinations were used to assess the acute toxicity of tetrodotoxin. Results According to the simulation calculation of Origin 8.0 software, the MNLC was 8.62 μmol/L and 15.2 μmol/L for LC10. Under the experimental conditions, tetrodotoxin at a concentration of 16.0 μmol/L and above induced pericardial edema and arrhythmia, leading to the death of zebra fish. The target organs for acute toxicity of tetrodotoxin were the heart and liver. The main manifestations were pericardial edema, arrhythmia, and delayed yolk sac absorption. The toxicity appeared at a concentration of 0.958 μmol/L. Conclusion Tetrodotoxin has heart and liver toxicity to zebra fish, and its toxicity is dose-dependent. |
| keywords:tetrodotoxin zebra fish acute toxicity |
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