雌激素对糖尿病大鼠内皮祖细胞功能的影响及其机制研究
投稿时间:2021-06-23  修订日期:2021-10-25  点此下载全文
引用本文:董雅芬,王建,李莉,李姝姝,刘河龙,邱彦.雌激素对糖尿病大鼠内皮祖细胞功能的影响及其机制研究[J].药学实践杂志,2022,40(4):320~325
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作者单位E-mail
董雅芬 上海市浦东新区人民医院, 上海 201200  
王建 上海市浦东新区人民医院, 上海 201200  
李莉 上海市浦东新区人民医院, 上海 201200  
李姝姝 上海市浦东新区人民医院, 上海 201200  
刘河龙 上海市浦东新区人民医院, 上海 201200  
邱彦 上海市浦东新区人民医院, 上海 201200 qiuyan2189@163.com 
基金项目:上海市浦东新区人民医院科技项目(E17-04);浦东新区卫生健康委员会领先人才培养项目(PRWL2020-08)
中文摘要:目的 探索雌激素对糖尿病大鼠内皮祖细胞(EPCs)功能的影响及其可能的作用机制。方法 取健康Wistar大鼠骨髓提取EPCs并采用流式细胞仪和荧光显微镜鉴定。大鼠给予链脲佐菌素诱导为糖尿病模型,提取正常大鼠和糖尿病大鼠骨髓EPCs并培养,糖尿病大鼠EPCs体外给予雌激素10 nmol/L孵育24 h。检测EPCs增殖和功能;测定EPCs中锰超氧化物歧化酶(MnSOD)水平和NO水平及上清液中凝血酶敏感蛋白-1(TSP-1)蛋白水平。结果 与对照组比较,糖尿病EPCs的细胞增殖能力、迁移能力和小管形成功能受损(P<0.01),而雌激素体外干预后细胞增殖能力、迁移能力和小管形成功能均得到改善(P<0.01);糖尿病EPCs中MnSOD水平和NO水平明显下调,上清液中TSP-1蛋白水平升高(P<0.01);雌激素孵育能逆转上述改变(P<0.01)。结论 雌激素能改善糖尿病大鼠EPCs迁移能力和小管形成功能,作用机制可能与其降低糖尿病EPCs内的氧化应激及下调TSP-1的表达相关。
中文关键词:雌激素  内皮祖细胞  糖尿病  凝血酶敏感蛋白  锰超氧化物歧化酶  一氧化氮
 
Effect and mechanism of estrogen on EPCs function in diabetic rats
Abstract:Objective To explore the effect and mechanism of estrogen on endothelial progenitor cells(EPCs)function in diabetic rats. Methods EPCs were isolated from bone marrow of rats and characterized by fluorescence microscopy and flow cytometry. Rat diabetic model was established via streptozotocin induction. The bone marrow was taken to culture EPCs. EPCs of diabetes were incubated with Estrogen 10 nmol/L for 24h. The functions and proliferation of EPCs in vitro were detected. The levels of MnSOD and NO in EPCs and TSP-1 in supernatant were assayed. Results Compared with control group, EPCs proliferation, adhesion and angiogenesis functions were impaired in diabetic rats. The level of MnSOD and NO in diabetic EPCs were significantly decreased, while TSP-1 protein level in the supernatant increased. The above changes can be reversed with estrogen incubation. Conclusion Estrogen improved the EPCs migration and tubule formation in diabetic rats. The mechanism may be related to the reduction of oxidative stress and downregulation of TSP-1 expression in diabetic EPCs.
keywords:estrogen  endothelial progenitor cells  diabetes  TSP-1  MnSOD  NO
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