| 海绵共附生土曲霉的化学成分研究 |
| 投稿时间：2021-07-07 修订日期：2021-10-14 点此下载全文 |
| 引用本文：于熙,王玉婷,林厚文,孙雅婷.海绵共附生土曲霉的化学成分研究[J].药学实践杂志,2022,40(2):120~124 |
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| 基金项目:沈阳医学院博士科研启动基金（20195073） |
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| 中文摘要:目的 对海绵共附生真菌土曲霉Aspergillus terreus中的化学成分进行研究。方法 采用葡聚糖凝胶柱色谱、硅胶柱色谱和高效液相色谱等手段分离纯化；通过波谱数据鉴定化合物结构；采用PNPG法和DPPH法分别对分离得到的化合物进行α-葡萄糖苷酶抑制活性和抗氧化活性测试。结果 从土曲霉Aspergillus terreus中分离得到8个化合物，分别鉴定为methyl-3,4,5-trimethoxy-2-(2-(nicotinamido) benzamido) benzoate (1)、terrelumamide A (2)、emeheterone (3)、(8R,9S)-dihydroisoflavipucine (4)、(8S,9S)-dihydroisoflavipucine (5)、cyclo(S-Pro-S-Phe) (6)、brevianamide F (7)、terrein (8)。活性测试结果表明，化合物3具有较强的α-葡萄糖苷酶的抑制活性，IC50值为14.28 μmol/L。结论 化合物3、4、5、7为首次从土曲霉Aspergillus terreus中分离得到。 |
| 中文关键词:土曲霉 化学成分 α-葡萄糖苷酶抑制活性 |
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| Study on chemical constituents of sponge-associated Aspergillus terreus |
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| Abstract:Objective To study the chemical constituents of Aspergillus terreus from sponge epiphytic fungal. Methods Sephadex LH-20 column chromatography, silica gel column chromatography and high performance liquid chroma-tography were used to separate and purify the compounds. The structures of compounds were identified by spectroscopic data. The α-glucosidase inhibitory activity and antioxidant activity of the compounds were tested by PNPG and DPPH methods, respectively. Results Eight compounds were isolated from Aspergillus terreus and identified as methyl-3,4,5-trimethoxy-2-(2-(nicotinamido) benzamido) benzoate (1), terrelumamide A (2), emeheterone (3), (8R,9S)-dihydroisoflavipucine (4), (8S,9S)-dihydroisoflavipucine (5), cyclo(S-Pro-S-Phe) (6), brevianamide F (7), terrein (8). Compound 3 showed strong inhibitory activity against α-glucosidase and the IC50 value was 14.28 μmol/L. Conclusion Compounds 3, 4, 5, and 7 were obtained from Aspergillus terreus for the first time. |
| keywords: Aspergillus terreus chemical constituent α-glucosidase inhibitory activity. |
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