| UHPLC法测定辐射后的酒石酸唑吡坦片含量 |
| 投稿时间：2021-10-11 修订日期：2022-01-04 点此下载全文 |
| 引用本文：张稳,黄心慧,杨星瑞,周婷婷,高建义,李勇枝.UHPLC法测定辐射后的酒石酸唑吡坦片含量[J].药学实践杂志,2022,40(1):62~65 |
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| 基金项目:军队后勤科研项目（BWS17J028） |
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| 中文摘要:目的 建立UHPLC法测定辐射后的酒石酸唑吡坦片含量，考察不同辐射剂量对酒石酸唑吡坦片含量的影响。方法 采用超高效液相色谱法，对γ射线辐射的酒石酸唑吡坦片进行含量测定。采用C18柱以乙腈-甲醇-0. 05 mol/L磷酸溶液（用三乙胺调节pH值至5.5）（18∶26∶56）为流动相，流速为0.7 ml/min，检测波长为254 nm。结果 酒石酸唑吡坦浓度在5～80 μg/ml范围内线性良好，r=0.9996；平均加样回收率为98.2%，RSD为1.72%，重复性为0.87%。0、8、25和80 kGy辐射量下酒石酸唑吡坦含量分别为105.1%、106.4%、102.7%和105.4%。结论 超高效液相色谱法分析周期短、结果准确，适用于辐射后酒石酸唑吡坦片的含量测定，辐射后酒石酸唑吡坦片含量基本保持不变。 |
| 中文关键词:酒石酸唑吡坦 超高效液相色谱法 含量测定 辐射 |
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| Determination of zolpidem tartrate tablets after radiation by UHPLC |
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| Abstract:Objective To establish a UHPLC method for the determination of zolpidem tartrate tablets after radiation, and to investigate the effect of different radiation doses on the content of zolpidem tartrate tablets. Methods Ultra high performance liquid chromatography was used. The content of zolpidem tartrate tablets irradiated by γ-ray was determined. Using C18 column, acetonitrile methanol-0.05 mol/L phosphoric acid solution (the pH value as 5.5 with triethylamine) (18∶26∶56) was used as the mobile phase. The flow rate was 0.7 ml/min, and the detection wavelength was 254 nm.Results The method validation showed good linearity in the concentration range of 5-80 μg/ml (r=0.999 6); The average recovery was 98.2%, RSD was 1.72%, and the repeatability was 0.87%. The contents of zolpidem tartrate were 105.1%, 106.4%, 102.7% and 105.4% under 0, 8, 25 and 80 kGy radiation.Conclusion UHPLC has accurate results with short analysis cycle in this study. It is suitable for the determination of zolpidem tartrate tablets after radiation. The content of zolpidem tartrate tablets remained basically unchanged after radiation. |
| keywords:zolpidem tartrate ultra high performance liquid chromatography content determination radiation |
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