| HPLC-ELSD法同时测定知母药材中5种成分的含量 |
| 投稿时间：2021-09-13 修订日期：2022-01-05 点此下载全文 |
| 引用本文：戴建英,尤巍,田甜,洪战英.HPLC-ELSD法同时测定知母药材中5种成分的含量[J].药学实践杂志,2022,40(1):34~37 |
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| 基金项目:国家自然科学基金项目（81673386、81872829） |
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| 中文摘要:目的 采用高效液相色谱-蒸发光散射检测方法（HPLC-ELSD）建立同时测定知母药材中新芒果苷、芒果苷、知母皂苷BⅡ、宝藿苷Ⅰ及知母皂苷AⅢ的含量测定方法。方法 采用Agilent poroshell 120 EC-C18柱，流动相采用乙腈-0.2%醋酸水系统，梯度洗脱；柱温为30 ℃，流速为0.7 ml/min；蒸发光散射检测器以氮气为雾化气，雾化气温度为40 ℃，漂移管温度为90 ℃，氮气体积流量为2.0 L/min；进样量为20 μl。结果 5种成分均能达到基线分离，新芒果苷24.1～386 μg/ml（r=0.999 3）、芒果苷23.2～371 μg/ml（r=0.998 6）、知母皂苷BⅡ 54.2～867.2 μg/ml（r=0.995 6）、宝藿苷Ⅰ 5.3～84.8 μg/ml（r=0.996 8）、知母皂苷AⅢ 10～160 μg/ml（r=0.998 9）的浓度范围内呈现良好的线性关系。5种成分的平均加样回收率在101.8%～105.0%之间，重复性RSD小于2.4%，知母药材中上述5种成分含量分别为1.62%、0.82%、7.36%、0.07%、0.34%。结论 该方法操作简便、准确、灵敏度高，可用于知母药材的多指标成分定量测定。 |
| 中文关键词:高效液相色谱-蒸发光散射检测法 知母 含量测定 |
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| Simultaneous determination of five components in Anemarrhenae Rhizoma by HPLC-ELSD |
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| Abstract:Objective To establish a method for the simultaneous determination of new mangiferin, mangiferin, artemisinin BⅡ, icariin and artemisinin A in Anemarrhenae Rhizoma by high performance liquid chromatography-evaporation light scattering detector (HPLC-ELSD). Methods The column was Agilent Poroshell 120 EC-C18. The mobile phase used acetonitrile-0.2% acetic acid water system with gradient elution. Column temperature was 30 ℃. Flow rate was 0.7 ml/min. Evaporative light scattering detector used nitrogen as atomizing gas. The atomizing gas temperature was 40 ℃ and the drift tube temperature was 90 ℃. The nitrogen volume flow rate was 2.00 L/min and the sample volume was 20 μl. Results The five components were able to achieve baseline separation. Neomangiferin, Mangiferin, Anemaponin BⅡ, Baohuoside I , Anemarrhena saponin AⅢwere determined as 24.1-386 μg/ml (r=0.999 3), 23.2-371 μg/ml (r=0.998 6), 54.2-867.2 μg/ml(r=0.995 6), 5.3-84.8 μg/ml (r=0.996 8), 10-160 μg/ml (r=0.998 9) respectively, which showed a good linear relationship within the concentration range. The average recovery rate of the five components was between 101.8% and 105.0%, and the repeatability RSD was less than 2.4%. The content of the above five components in Zhimu medicinal materials were 1.62%, 0.82%, 7.36%, 0.07%, 0.34%, respectively. Conclusion The method is simple, accurate, and highly sensitive, which could be used as the quantitative determination of multiple index components of Anemarrhenae Rhizoma. |
| keywords:high performance liquid chromatography-evaporative light scattering detector Anemarrhenae Rhizoma content determination |
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