| 不同产地藤茶中二氢杨梅素含量及其与牛蒡子配伍的药效学研究 |
| 投稿时间：2021-03-27 修订日期：2021-05-06 点此下载全文 |
| 引用本文：孙睿,付丽,雷雨.不同产地藤茶中二氢杨梅素含量及其与牛蒡子配伍的药效学研究[J].药学实践杂志,2021,39(3):255~258 |
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| 基金项目:国家重点研发计划（2018YFC1706803） |
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| 中文摘要:目的 探究不同产地、部位及加工工艺藤茶中二氢杨梅素含量的差异，及优选藤茶与牛蒡子配伍药效学研究。方法 二氢杨梅素高效液相方法学验证采用Agilent ZORBAX SB-C18柱，流动相为甲醇-0.05 % 磷酸（30∶70），流速为1 ml/min，检测波长为291 nm，柱温25 ℃。配伍药效验证方法采用大鼠棉球植入致炎及小鼠耳肿胀致炎模型，对大鼠棉球植入实验肉芽肿净量及小鼠耳肿胀率进行观察。结果 方法学验证二氢杨梅素在0.019 9～0.318 mg/ml范围内线性关系良好（r=0.999），回收率在95.04 %～100.4 %之间，样品在24 h内稳定，该方法重复性较好。配伍药效学验证高剂量优选藤茶与牛蒡子配伍可致大鼠肉芽肿净量及小鼠耳肿胀率均显著低于空白对照组。结论 方法简便准确，二氢杨梅素在不同产地、部位及加工工艺中含量差异较大，其中以贵州省江口县自然晒干的芽尖部位藤茶含量最高。藤茶与牛蒡子配伍可显著改善咽部症状，减轻致炎程度，共同协同达到清咽效果。 |
| 中文关键词:清咽 藤茶 牛蒡子 二氢杨梅素 高效液相色谱 |
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| Therapeutic effects study on the contents of dihydromyricetin in ampelopsis grossedentata from different places and their compatibility with fructus arctiine |
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| Abstract:Objective To study the content differences of Dihydromyricetin in Ampelopsis grossedentata from different origins, parts and processing techniques, and improve the therapeutic effects by optimizing the compatibility of Ampelopsis grossedentata with Fructus arctiine.Methods The HPLC separation of Dihydromyricetin was performed on an Agilent ZORBAX SB-C18 column with methanol, 0.05 % phosphoric acid (30∶70) as the mobile phase. The flow rate was 1 ml/min, the detection wavelength was 291 nm, and the column temperature was 25 ℃. Compatibility efficacy verification was performed with the inflammation model caused by cotton ball implantation in rats and ear swelling in mice. The net granulomatosis in the rats with cotton ball implantation and the swelling rate of mouse ears were recorded.Results Dihydromyricetin had a good liner recovery between 0.019 9-0.318 mg/ml (r=0.999). The extracted recovery was in the range of 95.04 %-100.4 %. The sample was stable within 24 h. This method had good repeatability. The combination of optimized high-dose Ampelopsis grossedentata with Fructus arctiine resulted in significantly lower net granuloma in rats and ear swelling rate in mice compared to the blank control group.Conclusion This method is simple and accurate. The content of dihydromyricetin varies greatly with different origins, parts, and processing techniques. Among them, the natural sun-dried vine tea in Jiangkou County, Guizhou Province has the highest content. The combination use of Ampelopsis grossedentata and Fructus Arctiine can significantly alleviate the pharyngeal symptoms, reduce the degree of inflammation, and achieve the therapeutic effect of clearing pharynx. |
| keywords:throat clearing Ampelopsis grossedentata Fructus arctiine Dihydromyricetin HPLC |
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