UHPLC-MS/MS法测定新冠肺炎经验方寒湿郁肺汤中11种主成分的含量
投稿时间:2020-12-05  修订日期:2021-03-13  点此下载全文
引用本文:崔莉莉,陈勇,张蒙伟,鄢红霞,高守红,陈万生.UHPLC-MS/MS法测定新冠肺炎经验方寒湿郁肺汤中11种主成分的含量[J].药学实践杂志,2021,39(2):148~151,156
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作者单位E-mail
崔莉莉 上海长征医院药学部上海 200000
宜春学院江西 宜春 336000 
 
陈勇 上海长征医院药学部上海 200000  
张蒙伟 上海长征医院药学部上海 200000
宜春学院江西 宜春 336000 
 
鄢红霞 上海长征医院药学部上海 200000
云南中医药大学云南 昆明 650000 
 
高守红 上海长征医院药学部上海 200000 gaoshouhong@smmu.edu.cn 
陈万生 上海长征医院药学部上海 200000  
基金项目:上海市临床药学重点专科建设项目(2016-40044-002);上海市卫生计生系统重要薄弱学科建设项目(2016ZB0303)
中文摘要:目的 建立测定寒湿郁肺汤中11个主成分含量的方法。方法 采用超高效液相色谱串联质谱法(UHPLC-MS/MS),色谱柱为Agilent ZORBAX SB-C18(3.5 μm,2.1 mm×150 mm ),流动相为0.2%甲酸-10 mmol/L醋酸铵水溶液(A)-乙腈(B),梯度洗脱(0~0.6 min, 80%~40%A; 0.6~1 min, 40%~30%A; 1~4.3 min, 30%~5%A),流速0.3 ml/min,柱温40 ℃。以利血平为检测内标,采用多反应监测模式同时测定香草酸、厚朴酚、和厚朴酚、汉黄芩素、刺槐素、6-姜酚、桔皮素、羌活醇、川陈皮素、紫花前胡苷、橙皮苷11个化学主成分的含量。结果 寒湿郁肺汤中11个主成分在各自浓度范围内线性关系良好(r>0.98),平均回收率在93.11%~111.73%。结论 该实验所建立的方法操作简便、重复性好,为寒湿郁肺汤的质量控制提供了实验室依据。
中文关键词:超高效液相色谱串联质谱法  含量测定  新型冠状病毒肺炎  寒湿郁肺汤
 
Determination of 11 main components in Hanshi Yufei decoction, a COVID-19 empirical prescription by UHPLC-MS/MS
Abstract:Objective To establish a method to determine 11 main components in Hanshi yufei decoction.Methods The method adopted UHPLC-MS/MS with an Agilent ZORBAX SB-C18 (3.5 μm,2.1 mm×150 mm) column. The mobile phase was consisted of 0.2% formic acid plus 10 mmol/L ammonium acetate aqueous solution(A) - acetonitrile(B) and gradient elution (0–0.6 min, 80%–40%A; 0.6–1 min, 40%–30%A; 1–4.3 min, 30%–5%A) at 0.3 ml/min. The column temperature was 40 ℃ and 11 main components including vanillic acid, magnolol, honokiol, wogonin, sophorin, 6-gingerol, citrinin, qianghuo alcohol, nobiletin, nodakenin, and hesperidin were quantified in a multiple reaction monitoring mode. The reserpine was the standard.Results The 11 main components in Hanshi Yufei decoction had a good linear relationship within their concentration range (r>0.98), and the average recovery was 93.11%~111.73%.Conclusion The UHPLC-MS/MS method established in this experiment is easy to operate and has good reproducibility, which provides a laboratory basis for the quality control of Hanshi Yufei decoction.
keywords:UHPLC-MS/MS  content determination  COVID-19  Hanshi Yufei decoction
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