益母草碱抑制NLRP3炎症小体过度激活调控巨噬细胞M1/M2表型分化
投稿时间:2021-01-03  修订日期:2021-03-07  点此下载全文
引用本文:华荣,陈瑶.益母草碱抑制NLRP3炎症小体过度激活调控巨噬细胞M1/M2表型分化[J].药学实践杂志,2021,39(2):143~147
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作者单位
华荣 泰州市人民医院药学部临床药学科江苏 泰州 225300 
陈瑶 泰州市人民医院药学部临床药学科江苏 泰州 225300 
中文摘要:目的 研究益母草碱对脂多糖(LPS)诱导小鼠腹腔巨噬细胞免疫应答影响及相关机制。方法 分离小鼠腹腔巨噬细胞,用脂多糖和益母草碱预处理24 h,MMT法检测巨噬细胞活性;Griess法检测NO释放量;ELISA法检测IL-1β、IL-18、IL-6、TNF-α的释放量;RT-PCR法检测NLRP3、ASC、caspase-1、TNF-α、iNOS、Arg-1和CD206的mRNA表达量;Western blot检测NLRP3、ASC、caspase-1蛋白表达量。结果 益母草碱能显著抑制脂多糖引起的巨噬细胞上清液中NO、IL-1β、IL-18、IL-6、TNF-α的释放。RT-PCR及Western blot实验结果显示,益母草碱可以抑制脂多糖引起的巨噬细胞中NLRP3、ASC、caspase-1的mRNA及蛋白表达;益母草碱还能明显抑制脂多糖所诱导的巨噬细胞向M1型分化,并促进巨噬细胞向M2型分化。结论 益母草碱能通过抑制NLRP3炎症小体,促进脂多糖诱导的巨噬细胞由M1表型向M2表型分化。
中文关键词:益母草碱  脂多糖  巨噬细胞  NLRP3炎症小体  M1/M2表型
 
Effect of leonurine on peritoneal macrophages M1/M2 phenotypic differentiation via inhibiting overactivation of NLRP3 inflammasome
Abstract:Objective To find the effect of leonurine on LPS-induced macrophages activation and its potential mechanism.Methods Mouse primary peritoneal macrophages were isolated and pretreated for 24 h with LPS and leonurine. MTT assay was used to detect the cell viability of macrophages. The production of IL-1β, IL-6, TNF-α and IL-18 in culture medium were tested by ELISA, and the production of NO was detected by Griess reagent. The mRNA expression of NLRP3, ASC, caspase-1, TNF-α, iNOS, Arg-1 and CD206 were detected by RT-PCR, and the protein expression of NLRP3, ASC and caspase-1 were detected by Western blotting.Results LPS can significantly increase the releases of NO、IL-1β、IL-6、TNF-α and IL-18 from macrophages. Leonurine can suppress the expression of pro-inflammatory factor levels, such as IL-1β (P<0.05), IL-18 (P<0.05), NO(P<0.05), IL-6(P<0.05) and TNF-α (P<0.05). Leonurine can decrease the activation of macrophage as well as the expression of NLRP3 Inflammasome.Protein expressions of NLRP3、ASC、caspase-1 were mitigated.Conclution Leonurine exerts beneficial effects through M1/M2 phenotypic differentiation of peritoneal macrophage via inhibiting overactivation of NLRP3 inflammasome. These findings suggest that leonurine might have a therapeutic potential for pelvic inflammatory disease.
keywords:macrophage  inflammasome  leonurine  NLRP3 inflammasome  M1/M2 polarization
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