| 化学迷路切除对小鼠应激镇痛的影响 |
| 投稿时间：2020-12-02 修订日期：2021-02-26 点此下载全文 |
| 引用本文：栗志远,周铎,闫琼,王新红.化学迷路切除对小鼠应激镇痛的影响[J].药学实践杂志,2021,39(2):138~142 |
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| 基金项目:青海省科技计划项目（2014-ZJ-753） |
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| 中文摘要:目的 探讨前庭器官在旋转诱发的小鼠应激镇痛中的作用。方法 雌性小鼠随机分为吗啡组和旋转组，每组小鼠腹腔注射纳洛酮或生理盐水15 min后，观察给予吗啡或旋转（转速250 r/min，时间1 min，每旋转15 s暂停5 s）刺激后的热板潜伏期。另取小鼠连续7 d皮下注射吗啡形成耐受后，观察吗啡耐受小鼠旋转后的热板潜伏期。最后，内耳注射对氨基苯胂酸损伤小鼠前庭器官，观察化学迷路切除小鼠旋转后的热板潜伏期。结果 与生理盐水组比较，纳洛酮组小鼠旋转后热板潜伏期无明显变化（P>0.05），皮下注射吗啡后热板潜伏期显著下降（P<0.05）。吗啡耐受小鼠旋转后热板潜伏期与生理盐水组比较无明显变化（P>0.05）。内耳注射对氨基苯胂酸后，小鼠接触翻正反射恢复时间显著增加、游泳能力显著下降（P<0.05），且化学迷路切除小鼠旋转后热板潜伏期显著缩短（P<0.05）。结论 化学迷路切除完全阻滞了旋转诱发的小鼠应激镇痛。前庭器官在旋转诱发的应激镇痛中起重要作用，且该应激镇痛可能由非阿片系统介导。 |
| 中文关键词:旋转 应激镇痛 吗啡 前庭器官 |
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| Effects of chemical labyrinthectomy on stress analgesia induced by rotation in mice |
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| Abstract:Objective To investigate the role of vestibular organs on stress analgesia induced by rotation in mice.Methods Female mice were randomly divided into morphine group and rotation group. After 15 minutes of intraperitoneal injection of naloxone or normal saline, the hot plate latency of mice in each group was observed following morphine injection or rotation (250 r/min, 15 s on with 5 s off). After subcutaneous injecting morphine for 7 consecutive days, tolerance was formed and the hot plate latency in morphine-tolerant mice after rotation was observed. P-aminophenylarsonic acid was injected into the inner ear to damage the vestibular organs of the mice and the hot plate latency was observed in chemically labyrinthectomy mice.Results Compared with the normal saline group, the hot plate latency of mice in the naloxone group did not change significantly after rotation (P>0.05), and the hot plate latency decreased significantly after subcutaneous injection of morphine (P<0.05). The morphine-tolerant mice had no significant change in the hot plate latency after rotation compared with the normal saline group (P>0.05). After injection of p-aminophenylarsonic acid into the inner ear, the recovery time of the righting reflex in mice was significantly increased, and the swimming ability was significantly reduced (P<0.05), and the hot plate latency of mice with chemical labyrinthectomy was significantly shortened after rotation (P<0.05).Conclusion Chemical labyrinthectomy completely blocked the rotation-induced stress analgesia in mice. Vestibular organs play an important role in rotation-induced stress analgesia, and this stress analgesia may be mediated by a non-opioid system. |
| keywords:rotation morphine stress-induced analgesia vestibular organs |
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