烟酰胺单核苷酸对内毒素休克小鼠死亡率的影响
投稿时间:2021-02-05  修订日期:2021-03-10  点此下载全文
引用本文:汪东昇,郑斯莉,程明和,缪朝玉.烟酰胺单核苷酸对内毒素休克小鼠死亡率的影响[J].药学实践杂志,2021,39(2):134~137,142
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作者单位E-mail
汪东昇 海军军医大学药理学教研室上海 200433  
郑斯莉 海军军医大学药理学教研室上海 200433  
程明和 海军军医大学药理学教研室上海 200433  
缪朝玉 海军军医大学药理学教研室上海 200433 cymiao@smmu.edu.cn 
基金项目:上海市科委生物医药领域科技支撑项目(16431901400);国家自然科学基金重点项目(81730098)
中文摘要:目的 探究烟酰胺单核苷酸(NMN)对脂多糖(LPS)诱导的内毒素休克小鼠死亡率的影响。方法 将10周龄C57BL/6J雄性小鼠随机分组,均腹腔注射LPS(10 mg/kg)造模。NMN腹腔注射给药,分为3种方式:①造模后0.5 h给药,剂量为10、30、100、300 mg/kg;②造模前0.5 h给药,剂量为30、100、300、600 mg/kg;③造模后0.5 h和12 h两次给药,每次300 mg/kg。观察记录每组小鼠的死亡情况,并绘制生存曲线。结果 与溶剂对照组相比,造模后0.5 h或造模前0.5 h给予不同剂量NMN,均不能改善小鼠死亡率或延缓死亡时间;造模后0.5 h和12 h两次给予NMN会加速小鼠死亡,增加小鼠死亡率。两个厂家的NMN产品效果类似。结论 NMN对LPS诱导的内毒素休克小鼠不具有治疗作用,小鼠内毒素休克发生后进行NMN多次给药会增加死亡率。
中文关键词:烟酰胺单核苷酸  脂多糖  内毒素休克  死亡率
 
Effect of nicotinamide mononucleotide on mortality of mice with endotoxic shock
Abstract:Objective To study the effect of nicotinamide mononucleotide (NMN) on the mortality of the lipopoly-saccharide (LPS)-induced endotoxic shock mouse model.Methods 10-week-old C57BL/6J male mice were randomly divided into groups, and were injected intraperitoneally (i.p.) with LPS (10 mg/kg) to induce endotoxic shock models. NMN was i.p. injected in three ways: (1) 0.5 h after modeling, doses of 10, 30, 100 and 300 mg/kg; (2) 0.5 h before modeling, doses of 30, 100, 300 and 600 mg/kg; or (3) 0.5 and 12 h after modeling, dose of 300 mg/kg each time. The death times of each group were recorded, and the survival curves were drawn.Results Compared with the solvent control group, NMN at different doses given 0.5 h after or before modeling didn’t improve the survival rate or delay the death time of endotoxic shock mice; But when given at 0.5 and 12 h 300 mg/kg after modeling, NMN accelerated the death of mice and increased the mortality of mice. NMN products by two manufacturers showed similar effects.Conclusion NMN has no therapeutic effect on LPS-induced endotoxic shock, and repeated administration of NMN after endotoxic shock will increase the mortality.
keywords:nicotinamide mononucleotide  lipopolysaccharide  endotoxic shock  mortality
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