动脉粥样硬化高脂饲料对小鼠糖脂水平的作用研究
投稿时间:2020-12-02  修订日期:2021-01-24  点此下载全文
引用本文:王品,齐齐,郑斯莉,徐浩展,缪朝玉.动脉粥样硬化高脂饲料对小鼠糖脂水平的作用研究[J].药学实践杂志,2021,39(2):121~125
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作者单位E-mail
王品 海军军医大学药理学教研室上海 200433  
齐齐 海军军医大学药理学教研室上海 200433  
郑斯莉 海军军医大学药理学教研室上海 200433  
徐浩展 海军军医大学药理学教研室上海 200433  
缪朝玉 海军军医大学药理学教研室上海 200433 cymiao@smmu.edu.cn 
基金项目:国家自然科学基金资助项目(81730098)
中文摘要:目的 研究动脉粥样硬化高脂饲料对小鼠体重、血糖血脂水平及动脉粥样硬化斑块形成的作用,并且明确禁食时间对血脂检测结果的影响。方法 对10周龄雄性ApoE基因敲除(ApoE-/-)小鼠分别给予高脂饲料及普通饲料,4个月后观察动脉粥样硬化斑块情况;对10周龄雄性C57BL/6J小鼠分别给予普通饲料4周、普通饲料2周+高脂饲料2周、高脂饲料4周,考察动物的体重、肝脏、血糖、血脂水平,并分析正常饮食条件下取材前禁食12、6 h及不禁食条件下对血脂检测结果的影响。结果 给予高脂饲料的ApoE-/-小鼠动脉斑块面积显著增加(P<0.01);给予高脂饲料的C57BL/6J小鼠的体重增加(P<0.01),肝脏脂肪样变;血糖、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-c)、高密度脂蛋白胆固醇(HDL-c)水平显著升高(P<0.01),而三酰甘油(TG)水平明显下降(P<0.01)。相比于禁食12 h,禁食6 h及不禁食条件下检测到的三酰甘油水平明显升高(P<0.01)。结论 动脉粥样硬化高脂饲料可加速ApoE-/-小鼠形成动脉粥样硬化斑块,显著升高血糖、TC及LDL-c水平,但明显降低TG值。禁食时间可影响血清TG的检测结果。
中文关键词:高脂饲料  动脉粥样硬化  血糖  三酰甘油  胆固醇
 
Effect of atherosclerotic high-fat diet on the level of glucose and lipid in mice
Abstract:Objective To study the effects of atherosclerotic high-fat diet on body weight, blood glucose, blood lipid levels and atherosclerotic plaque formation in mice, and to determine the effect of fasting time on the results of blood lipid testing.Methods 10-week-old male ApoE knockout (ApoE-/-) mice were given high-fat diet and normal diet. The atherosclerotic plaques were observed four months later. 10 week old C57BL/6J male mice were given regular diet for 4 weeks, regular diet for 2 weeks + high-fat diet for two or four weeks. Body weight、liver、glucose, and the serum lipid levels were examined. The influence of fasting for 12 h, 6 h or no fasting on blood lipid detection results before sacrificing were studied.Results The atherosclerotic plaque area of ApoE-/- mice given high-fat diet increased significantly (P<0.01). C57BL/6J mice given high-fat diet gained weight (P<0.01). The glucose, TC, LDL-c and HDL-c were also increased in C57BL/6J mice with liver fat accumulation while the level of TG was significantly decreased(P<0.01). Compared with the fasting 12 h group, serum triglyceride (TG) was significantly increased (P<0.01)in fasting 6 h and no fasting groups.Conclusion The atherosclerotic high-fat diet can accelerate the formation of atherosclerotic plaques in ApoE-/- mice, significantly increase blood sugar, TC and LDL-c levels, but significantly reduce TG values.. The fasting time can affect serum triglyceride (TG) level.
keywords:high fat diet  atherosclerosis  glucose  triglyceride  cholesterol
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