微波消解-电感耦合等离子体原子发射光谱法测定全血中的镧
投稿时间:2020-12-10  修订日期:2020-12-07  点此下载全文
引用本文:张静如,华俊杰,韩凌,朱敏,鲁莹.微波消解-电感耦合等离子体原子发射光谱法测定全血中的镧[J].药学实践杂志,2021,39(2):118~120,125
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作者单位E-mail
张静如 海军军医大学药学院药剂学教研室上海 200433  
华俊杰 海军军医大学药学院药剂学教研室上海 200433  
韩凌 海军军医大学药学院药剂学教研室上海 200433  
朱敏 海军军医大学药学院药剂学教研室上海 200433  
鲁莹 海军军医大学药学院药剂学教研室上海 200433 acuace@163.com 
基金项目:国家科技重大专项(子课题)-长远航环境及应激损伤防治药物的临床前及临床研究(2018ZX09J18110-001)
中文摘要:目的 评估多效防护乳中镧(La)的透皮安全性,建立微波消解-电感耦合等离子体发射光谱(ICP-OES)法测定大鼠全血中镧的分析方法。方法 全血样品经微波消解后,采用电感耦合等离子发射光谱仪分析,以333.749 nm为分析谱线,对大鼠全血中的La进行含量测定。结果 该方法的标准曲线相关性良好(r>0.9994),方法检出限为0.0025 μg/ml,定量限为0.0077 μg/ml,精密度<3%,回收率在94.9%~102.0%之间。结论 基于微波消解的ICP-OES方法稳定可靠,可为镧元素的含量测定和给药安全性研究提供重要依据。
中文关键词:  微波消解  多效防护乳  电感等离子体原子发射光谱  透皮
 
Determination of lanthanum in whole blood by microwave digestion-inductively coupled plasma optical emission spectrometry
Abstract:Objective To evaluate the transdermal safety of lanthanum (La) in sunscreen and jellyfish sting protective lotion, establish a microwave digestion-inductively coupled plasma opticalemission spectroscopy (ICP-OES) method for determination of lanthanum (La) in rat’s whole blood.Methods The whole blood samples were digested by microwave and analyzed by inductively coupled plasma emission spectrometer (ICP-OES). Using 333.749 nm as the analysis line, the content of La in rat whole blood was determined.Results The correlation linearity of the standard curve of this method was good (r>0.9994), the detection limit of the method was 0.0025 μg/ml, the limit of quantification was 0.0077 μg/ml, the precision was less than 3%, and the recovery rate was between 94.9% and 102.0%.Conclusion The ICP-OES method based on microwave digestion is stable and reliable, and can provide an important basis for the study of the transdermal safety of lanthanum.
keywords:lanthanum  microwave digestion  sunscreen and jellyfish sting protective lotion  inductive plasma optical emission spectrometry  transdermal
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