| 梗阻性黄疸对患者体内右美托咪定药动学的影响 |
| 投稿时间：2020-03-12 修订日期：2020-06-08 点此下载全文 |
| 引用本文：段虎,李盛建,周艳卿,杨俊炜,赵亮,李群英.梗阻性黄疸对患者体内右美托咪定药动学的影响[J].药学实践杂志,2021,39(1):73~76 |
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| 中文摘要:目的 建立超高效液相色谱-串联质谱法测定人血浆右美托咪定的浓度，评价梗阻性黄疸对患者体内右美托咪定药动学参数的影响。方法 样品经液-液萃取后测定，色谱柱为Agilent Eclipse Plus C18，流动相为甲醇−0.1%甲酸水溶液，流速为0.2 ml/min，柱温为35 ℃，质谱检测采用动态多反应离子检测模式。结果 人血浆中右美托咪定在0.01～10.00 ng/ml浓度范围内线性关系良好，日内和日间精密度均小于15.00%，提取回收率为85.5%～93.1%，基质效应为91.2%～95.6%，样品在分析期间稳定性良好。与对照组相比，梗阻性黄疸患者体内右美托咪定药动学参数cmax、AUC(0−t)、AUC(0−∞)和Vz分别增加63.4%、78.9%、66.4%、82.5%；CLz降低42.1%。结论 该方法结果准确，灵敏度高，重现性好，适用于检测人血浆右美托咪定的浓度；梗阻性黄疸患者体内右美托咪定消除减慢。 |
| 中文关键词:超高效液相色谱-串联质谱 梗阻性黄疸 右美托咪定 药动学 |
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| Effect of obstructive jaundice on pharmacokinetics of dexmedetomidine in vivo |
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| Abstract:Objective To establish a UPLC-MS/MS method for the determination of dexmedetomidine in human plasma and investigate the effect of obstructive jaundice on pharmacokinetics of dexmedetomidine in vivo.Methods Samples were obtained by liquid-liquid extraction. Agilent Eclipse Plus C18 column was used for chromatograph with methanol and 0.1% formic acid-water solution as mobile phase. Flow rate was 0.2 ml/min. The column temperature was 35 ℃, and the MS detection was selected in MRM mode.Results The calibration curves of dexmedetomidine showed good linearity in the ranges of 0.01−10.00 ng/ml. The results of intra and inter-day precisions were both within 15%. The recovery rate was 85.5%−93.1%. Matrix effect was 91.2%−95.6%. Samples remained stable during analysis. Compared with the control group, cmax、AUC(0−t)、AUC(0−∞) and Vz of dexmedetomidine in the patients with obstructive jaundice were increased by 63.4%, 78.9, 66.4%, 82.5%, respectively (P<0.01). CLz was decreased by 42.1%.Conclusion This method is accurate, sensitive and reproducible. It is suitable for dexmedetomidine assay in human plasma. The elimination rate of dexmedetomidine is slower in obstructive jaundice. |
| keywords:UPLC-MS/MS obstructive jaundice dexmedetomidine pharmacokinetics |
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