口腔溃疡膜有效成分提取方法的考察及含量测定
投稿时间:2020-03-06  修订日期:2020-04-10  点此下载全文
引用本文:陈顺,崔莉莉,高守红,陆文铨,宋江丽,王志鹏.口腔溃疡膜有效成分提取方法的考察及含量测定[J].药学实践杂志,2020,38(5):466~468,480
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作者单位E-mail
陈顺 海军军医大学附属长征医院药学部上海 200003  
崔莉莉 海军军医大学附属长征医院药学部上海 200003
宜春学院化学与生物工程学院江西 宜春 336000 
 
高守红 海军军医大学附属长征医院药学部上海 200003  
陆文铨 海军军医大学附属长征医院药学部上海 200003  
宋江丽 海军军医大学附属长征医院药学部上海 200003  
王志鹏 海军军医大学附属长征医院药学部上海 200003 wangzhipeng@smmu.edu.cn 
中文摘要:目的 考察不同溶剂对口腔溃疡膜中有效成分地塞米松磷酸钠和克霉唑的提取率,优化有效成分含量测定方法。方法 采用不同提取溶剂(甲醇、水、70%甲醇)对口腔溃疡膜中的有效成分进行提取,用HPLC法进行含量测定,得到最佳提取方法。结果 纯水对口腔溃疡膜中地塞米松磷酸钠和克霉唑有最好的提取效果。地塞米松磷酸钠在0.482~16.328 μg/ml范围内呈良好的线性关系(r=0.999 9),平均回收率为(103.97±1.02)%;克霉唑在5.014 5~200.580 0 μg/ml范围内呈良好的线性关系(r=0.999 8),平均回收率为(104.23±0.63)%。结论 本研究建立的纯水提取方法操作简单,提取效率高,含量测定方法操作简便,准确性、重复性好。
中文关键词:口腔溃疡膜  地塞米松磷酸钠  克霉唑  含量测定  高效液相色谱
 
Investigation of the extraction method and content determination of the active components from oral ulcer film
Abstract:Objective To investigate the extraction methods for active components from oral ulcer film and optimize the determination methods of active components dexamethasone sodium phosphate and metronazole.Methods Different extraction solvents(methanol, water and 70% methanol aqueous) were applied to extract the active components dexamethasone sodium phosphate and metronazole from oral ulcer film, which contents were quantified by a HPLC method.Results the extraction solvent water had the best efficacy and more simpler compared to the other two solvents. Clotriazole showed a good linear relationship within 5.014 5-200.5800 μg/ml (r=0.999 8), and the average extraction recovery was (104.23±0.63)%, and for dexamethasone sodium phosphate, a good linear relationship was obtained in the range of 0.482-16.328 μg/ml (r=0.9999), and the average extraction recovery was (103.97±1.02)%.Conclusion The water extraction method established in this study was simple and efficient, which showed features of simplicity, accuracy and repeatable.
keywords:oral ulcer film  dexamethasone sodium phosphate  clotrimazole  content determination  HPLC
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