RP-HPLC法同时测定防暑清热饮中5种活性成分的含量
投稿时间:2020-02-20  修订日期:2020-04-03  点此下载全文
引用本文:李杰,杨育儒,王庆芬,陈锦珊.RP-HPLC法同时测定防暑清热饮中5种活性成分的含量[J].药学实践杂志,2020,38(5):462~465
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作者单位E-mail
李杰 中国人民解放军联勤保障部队第九〇九医院 / 厦门大学附属东南医院药剂科福建 漳州363000  
杨育儒 中国人民解放军联勤保障部队第九〇九医院 / 厦门大学附属东南医院药剂科福建 漳州363000  
王庆芬 中国人民解放军联勤保障部队第九〇九医院 / 厦门大学附属东南医院药剂科福建 漳州363000  
陈锦珊 中国人民解放军联勤保障部队第九〇九医院 / 厦门大学附属东南医院药剂科福建 漳州363000 cjs18659341758@163.com 
基金项目:医院青年苗圃基金(17Y019)
中文摘要:目的 建立RP-HPLC法同时测定防暑清热饮中绿原酸、木犀草素-7-O-β-D-葡糖苷、3,5-O-二咖啡酰奎宁酸、蒙花苷和广藿香酮含量的方法。方法 采用ZORBAX-SB-C18色谱柱(250 mm×4.6 mm,5 μm),以0.2%磷酸溶液(A)-乙腈(B)梯度洗脱,流速为1.0 ml/min,检测波长为327 nm,柱温为30 ℃。结果 绿原酸、木犀草素-7-O-β-D-葡糖苷、3,5-O-二咖啡酰奎宁酸、蒙花苷和广藿香酮线性关系良好(r≥0.999 6),平均加样回收率(RSD)分别为102.03%(1.63%)、102.38%(1.51%)、102.39%(1.23%)、103.14%(1.87%)和104.01%(2.33%)。结论 本实验建立的测定方法简单、准确、重复性好,能够有效控制该制剂的质量。
中文关键词:防暑清热饮  绿原酸  木犀草素-7-O-β-D-葡糖苷  3,5-O-二咖啡酰奎宁酸  蒙花苷  广藿香酮  高效液相色谱法
 
Simultaneous determination of five active components in Fangshu Qingre mixture by RP-HPLC
Abstract:Objective To establish a method for simultaneous determination of chlorogenic acid, luteolin-7-O-β-D-glucoside,3,5-dicaffeoylquinic acid, linarin and pogostone in Fangshu Qingre mixture by RP-HPLC.Methods ZORBAX-SB-C18 column(4.6 mm×250 mm, 5 μm) was used as the chromatographic column.The mobile phase was 0.2% formic acid water solution(A) and acetonitrile solution (B)with a gradient elution mode. The flow rate was 1.0 ml/min.The detective wavelength was 327 nm. The column temperature was 30 ℃.Results There were good linear relationships in the determination of chlorogenic acid, luteolin-7-O-β-D-glucoside, 3,5-dicaffeoylquinic acid, linarin and pogostone (r≥0.999 6), with the average recovery rate of 102.03%(1.63%), 102.38%(1.51%), 102.39%(1.23%), 103.14%(1.87%) and 104.01%(2.33%).Conclusion The method was simple and stable with a good reproducibility, which could be used as a quality control method for active compotents in Fangshu Qingre mixture.
keywords:Fangshu Qingre mixture  chlorogenic acid  luteolin-7-O-β-D-glucoside  3,5-dicaffeoylquinic acid  linarin  pogostone  HPLC
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