黄连提取物减轻脓毒症相关急性肾损伤的代谢组学研究
投稿时间:2020-03-19  修订日期:2020-05-18  点此下载全文
引用本文:郑约楠,邵国建,张一帆,邵玲就,周琪,司亚晨.黄连提取物减轻脓毒症相关急性肾损伤的代谢组学研究[J].药学实践杂志,2020,38(5):435~440
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作者单位E-mail
郑约楠 温州市中心医院肾内科浙江 温州 325000  
邵国建 温州市中心医院肾内科浙江 温州 325000  
张一帆 温州市中心医院肾内科浙江 温州 325000  
邵玲就 温州市中心医院肾内科浙江 温州 325000  
周琪 海军军医大学研究生院上海 200433 chsnsyc@163.com 
司亚晨 海军军医大学研究生院上海 200433  
基金项目:温州市科技局科研基金(Y20140287; Y20140747)
中文摘要:目的 探讨黄连提取物(Rhizoma Coptidis extracts,RCE)对脓毒症相关急性肾损伤的影响及潜在机制。方法 将C57BL/6小鼠分为假手术组、模型组以及治疗组3组;采用试剂盒检测血清肌酐(serum creatinine,Scr)及尿素氮(blood urea nitrogen,BUN)水平;采用气相色谱-质谱进行代谢组学分析。结果 模型组较假手术组Scr、BUN水平均上调;而治疗组较模型组均下调,差异均有统计学意义(P<0.05)。代谢组学分析共获得16个代谢物可能与脓毒症相关急性肾损伤过程有关,主要参与氨基酸代谢和糖代谢。在这16个代谢物中,8个代谢物水平在黄连提取物干预下发生回调。结论 黄连提取物可能通过改善脓毒症相关急性肾损伤的代谢物变化从而起到治疗作用。
中文关键词:脓毒症  急性肾损伤  黄连  代谢组学
 
The protective effect of Rhizoma Coptidis extracts against the sepsis associated with acute kidney injury based on metabolic analysis
Abstract:Objective To investigate the potential mechanism of Rhizoma Coptidis extracts (RCE) against sepsis associated with acute kidney injury.Methods C57BL/6 mice were divided into sham group, model group and RCE treatment group. The levels of Scr and BUN were measured by test kits. Gas chromatography-mass spectrometry was used to analyze metabolic changes in kidneys.Results The levels of Scr and BUN were increased in the model group than sham, which were reversed by RCE. 16 metabolites related to the progress of sepsis associated with acute kidney injury were detected, which were involved in amino acid metabolism and carbohydrate metabolism. Among these metabolites, the level of 8 metabolites can be reversed with RCE treatment.Conclusion RCE might exert therapeutic effects in sepsis associated with acute kidney injury by altering multiple metabolic pathways.
keywords:sepsis  acute kidney injury  Rhizoma Coptidis  metabonomics
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