| 红色诺卡菌细胞壁骨架中化学成分的鉴别与单糖含量测定 |
| 投稿时间：2020-03-11 修订日期：2020-05-25 点此下载全文 |
| 引用本文：万众,于丹,王菲,李伟,张海.红色诺卡菌细胞壁骨架中化学成分的鉴别与单糖含量测定[J].药学实践杂志,2020,38(5):423~430 |
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| 基金项目:上海市卫健委科研面上项目（201740217） |
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| 中文摘要:目的 分析鉴别红色诺卡菌细胞壁骨架（Nr-CWS）中的化学成分，并对其中的单糖成分进行分析测定。方法 首先，采用UHPLC-Q-TOF/MS方法对Nr-CWS提取物中的化学成分进行分离与分析，通过与Metlin数据库中代谢物成分信息比对，快速鉴别其中化学成分。然后，对Nr-CWS提取物中多糖水解后衍生化，采用UHPLC-MS/MS法对单糖衍生物进行定量分析，测定单糖的含量。结果 共鉴别出Nr-CWS提取物中的64个化学成分，主要包括氨基酸、单糖、脂肪酸等成分。此外，建立了8种单糖柱前衍生化的UHPLC-MS/MS含量测定方法，含量测定结果表明，Nr-CWS中阿拉伯糖和半乳糖的含量最高，说明阿拉伯糖、半乳糖是组成Nr-CWS中多糖的主要组成成分。结论 通过对Nr-CWS中的主要化学成分进行了分离与分析，对多糖水解后的单糖成分进行含量测定，为今后开展Nr-CWS活性成分的筛选及药理作用机制的研究奠定基础。 |
| 中文关键词:红色诺卡菌细胞壁骨架 化学成分 鉴别 单糖 含量测定 |
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| Identification of chemical components and monosaccharide assay in Nocardia rubra cell wall skeleton |
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| Abstract:Objective To analyze and identify the chemical components in the Nocardia rubra cell wall skeleton (Nr-CWS), and to determine the contents of monosaccharides accurately.Methods The extract of Nr-CWS was separated and analyzed by UHPLC-Q-TOF/MS method. The chemical components were quickly identified by matching the data with the information in the Metlin database. The monosaccharide contents in the Nr-CWS extract were determined by UHPLC-MS/MS method after derivatization.Results A total of 64 chemical components were identified in the extract of Nr-CWS, including amino acids, monosaccharides and so on. A assay method for 8 monosaccharides by UHPLC-MS/MS was successfully established. The content of arabinose in Nr-CWS was the highest, followed by galactose, which indicated that the main polysaccharide components in Nr-CWS may be composed of these monosaccharides.Conclusion In this study, we analyzed the main chemical components of Nr-CWS, which are amino acids, fatty acids and so on. The content of monosaccharide after polysaccharide hydrolysis was determined by UHPLC-MS/MS. This will lay a foundation for the screening of the active components of Nr-CWS and the study of its pharmacological mechanism. |
| keywords:Nocardia rubra cell wall skeleton chemical component identification monosaccharide determination |
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