山奈酚对白假丝酵母生物被膜抑制作用的研究
投稿时间:2020-04-09  修订日期:2020-06-08  点此下载全文
引用本文:陈岚,沈娟,严万年,范凌志,曹颖瑛.山奈酚对白假丝酵母生物被膜抑制作用的研究[J].药学实践杂志,2020,38(5):413~417,430
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作者单位E-mail
陈岚 空军杭州特勤疗养中心疗养三区药械科浙江 杭州 310002  
沈娟 上海市皮肤病医院上海 200443  
严万年 上海市皮肤病医院上海 200443  
范凌志 上海市皮肤病医院上海 200443  
曹颖瑛 上海市皮肤病医院上海 200443 caoyingying608@163.com 
基金项目:国家自然科学基金面上项目(81671991)
中文摘要:目的 研究山奈酚抗白假丝酵母生物被膜的作用及其可能机制。方法 测定山奈酚对白假丝酵母处于形成过程中的生物被膜和成熟生物被膜代谢活性的影响;测定山奈酚对生物被膜基质产生水平的影响;显微镜下观察山奈酚对菌丝形成的抑制作用;水-烃两相分离法测定山奈酚对白假丝酵母细胞表面疏水性的影响;实时定量RT-PCR法测定山奈酚对生物被膜形成相关基因表达的影响。结果 山奈酚抑制白假丝酵母生物被膜形成,且呈剂量依赖性,同时具有抗成熟生物被膜作用,显著降低生物被膜基质含量;与对照组相比,山奈酚明显抑制白假丝酵母菌丝形成并降低其细胞表面疏水性;经山奈酚处理的白假丝酵母生物被膜形成相关基因BCR1NRG1TUP1的表达升高,同时HWP1EFG1CPH1ALS1ALS3CSH1的表达下降。结论 山奈酚具有抗白假丝酵母生物被膜活性,其机制与抑制菌丝形成及降低其细胞表面疏水性相关。
中文关键词:山奈酚  白假丝酵母  生物被膜  黏附  菌丝
 
Study on the antibiofilm activity of kaempferol in Candida albicans
Abstract:Objective To study the action of kaempferol (KAE) against Candida albicans biofilms and explore the potential mechanisms.Methods Biofilm metabolic activity assay was used to investigate the action of KAE against C. albicans biofilm formation as well as mature biofilm. The inhibition of KAE in hyphal formation was examined by microscope. The water-hydrocarbon two-phase separation assay was used to test the effect of KAE on the cell surface hydrophobicity of C. albicans. The mRNA expression of the genes involved in biofilm formation was determined by real time RT-PCR.Results KAE showed inhibition effect on C. albicans biofilm formation in a dose-dependent manner. Moreover, KAE inhibited mature biofilm. The biomass of biofilm was reduced upon KAE treatment. KAE inhibited hyphal formation and reduced the cell surface hydrophobicity of C. albicans. In the presence of KAE, the mRNA expression of the genes involved in biofilm formation was changed, with the up-regulation of BCR1,NRG1,TUP1 and down-regulation of HWP1,EFG1,CPH1,ALS1,ALS3 and CSH1.Conclusion KAE showed antifungal activity against C. albicans biofilm. The mechanisms may relate to the inhibition of hyphal formation and reduction of cell surface hydrophobicity.
keywords:kaempferol  Candida albicans  biofilm  adhesion  hyphae
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