| 硫化铜纳米粒的处方工艺优化与体外评价 |
| 投稿时间：2019-12-17 修订日期：2020-04-30 点此下载全文 |
| 引用本文：陈珍珍,陶春,张雪婷,周桂芝,张倩,宋洪涛.硫化铜纳米粒的处方工艺优化与体外评价[J].药学实践杂志,2020,38(4):328~333,345 |
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| 基金项目:福建省自然科学基金项目资助（2017J01822） |
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| 中文摘要:目的 为避免硫化铜（CuS）纳米粒体内蓄积，制备并优化CuS纳米粒，分析粒径影响因素并评价其光热性能。方法 在单因素考察基础上，采用星点设计-响应面法进一步分析各因素对CuS纳米粒粒径的影响，获得最佳处方工艺。考察优选的纳米粒的理化性质，包括形态、粒径稳定性、光热转换性能及光热稳定性。采用CCK-8法评价CuS纳米粒对4T1乳腺癌细胞以及HK2肾细胞的毒性，并考察其光热效应对4T1乳腺癌细胞的杀伤效果。结果 CuS纳米粒优选处方工艺的水合粒径为（10.53±1.63）nm；透射电镜下显示其粒径为（3.10±0.81）nm；所优选的CuS纳米粒具有良好的粒径稳定性，良好的光热转换性能与光热稳定性。细胞毒研究显示，所优选的CuS纳米粒分别在100 μg/ml及150 μg/ml浓度范围内，对4T1乳腺癌细胞以及HK2肾细胞均无显著的细胞毒性，且其对4T1乳腺癌细胞具有显著的光热杀伤效果。结论 所制备的CuS纳米粒实际粒径小于6 nm且具有良好的光热效应，有望解决CuS纳米粒体内蓄积的问题，使其更好地应用于抗肿瘤治疗。 |
| 中文关键词:硫化铜纳米粒 单因素考察 星点设计-响应面法 光热性能 |
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| Optimization of formulation process and in vitro evaluation of copper sulfide nanoparticles |
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| Abstract:Objective To avoid the accumulation of copper sulfide (CuS) nanoparticles, prepare and optimize CuS nanoparticles, analyze the factors affecting the particle size and evaluate their photothermal properties.Methods Based on the single factor study, central composite design-response surface methodology was used to optimize the CuS nanoparticle formulation process. The morphology, particle size stability, photothermal conversion efficiency, photothermal stability of optimized CuS nanoparticles were characterized. The toxicity of CuS nanoparticles on 4T1 breast cancer cells and HK2 kidney cells was evaluated by CCK-8 method. In vitro photothermal experiment was used to investigate the ability of CuS nanoparticles on killing 4T1 breast cancer cells.Results The average hydration dynamic diameter of optimized CuS nanoparticles was (10.53±1.63)nm, the actual particle size of CuS nanoparticles showed by TEM image was (3.10±0.81)nm. It had good particle size stability, good photothermal conversion efficiency and photothermal stability. Within the concentration range of 100 μg/ml and 150 μg/ml，it showed no significant toxicity on 4T1 breast cancer cells and HK2 kidney cells, indicating the good stability of CuS nanoparticles. In vitro photothermal therapy showed that CuS nanoparticles had good ability to kill 4T1 breast cancer cells by photothermal.Conclusion The prepared CuS nanoparticles have a small particle size (less than 6nm) and a good photothermal effect, which is expected to solve the problem of CuS nanoparticles accumulation in vivo and make it better for tumor treatment. |
| keywords:copper sulfide nanoparticles single factor study central composite design-response surface methodology photothermal effect |
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