| 白蔹药材中大黄素含量的反相高效液相色谱法测定 |
| 投稿时间：2019-11-26 修订日期：2020-01-03 点此下载全文 |
| 引用本文：孟飞,宋学立.白蔹药材中大黄素含量的反相高效液相色谱法测定[J].药学实践杂志,2020,38(3):264~267 |
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| 基金项目:原济南军区联勤部司令部课题（CJN15J052） |
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| 中文摘要:目的 建立白蔹药材中大黄素含量的测定方法，为完善白蔹药材的质量控制标准提供方法和依据。方法 采用反相高效液相色谱法，色谱柱：Apollo-C18柱（4.6 mm×250 mm，5 μm）；流动相：甲醇-0.2%磷酸（85:15）；流速：1.0 ml/min；柱温：室温；检测波长：220 nm。结果 在选定的色谱条件下，白蔹药材中大黄素在0.124～3.968 μg/ml范围内线性关系良好，直线回归方程为Y=53 962X–966.46，r=0.999 7，平均回收率为99.7%，RSD为2.5%（n=9）。结论 该方法所测结果准确、灵敏度高、重现性好，适用于白蔹药材中大黄素的含量测定。 |
| 中文关键词:白蔹 大黄素 高效液相色谱 |
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| Emodin assay in Ampelopsis radix by RP-HPLC |
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| Abstract:Objective To establish a method for Emodin assay in Ampelopsis radix, and to improve the quality control of Ampelopsis radix.Methods Reversed phase high performance liquid chromatography was used. The column: Apollo-C18 (4.6 mm×250 mm, 5 μm); mobile phase: methanol:0.2% phosphoric acid (85:15); flow rate: 1.0 ml/min; column temperature: room temperature; detection wavelength: 220 nm.Results With the specified chromatographic conditions, there was a good linear relationship for Emodin in Ampelopsis radix in the range of 0.124-3.968 μg/ml. The linear regression equation was Y= 53 962X–966.46, r=0.999 7. The average recovery was 99.7%. RSD=2.5% (n=9).Conclusion This method is accurate, sensitive and repeatable. It is suitable for the determination of emodin in Ampelopsis radix. |
| keywords:Ampelopsis radix emodin HPLC |
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