| 基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究 |
| 投稿时间：2018-05-05 修订日期：2018-11-13 点此下载全文 |
| 引用本文：范加腾,陆峰,许激扬.基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究[J].药学实践杂志,2019,37(2):156~161,169 |
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| 中文摘要:目的 建立动态表面增强拉曼光谱法（D-SERS）快速表征两性霉素B （AMB）对白色念珠菌的抑制作用。方法 采用D-SERS法，选用敏感的白色念珠菌分别与不同浓度的AMB相互作用，在不同的作用时间下，通过考察拉曼位移在656 cm-1和729 cm-1处的峰强比和药物浓度之间的关系，分析AMB对白色念珠菌拉曼光谱的影响。结果 在一定药物浓度和作用时间下，峰强比与AMB浓度呈负相关，可用于描述AMB对白色念珠菌的抑制作用，且药物浓度低于其最小抑菌浓度。结论 该方法简便、快捷，实现了AMB对白色念珠菌的快速表征，同时为表征更多药物-病原体相互作用提供了更简单、快速的检测方法。 |
| 中文关键词:白色念珠菌 两性霉素B 动态表面增强拉曼光谱法 |
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| Research on the inhibitory effect of amphotericin B against Candida albicans by D-SERS |
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| Abstract:Objective To develop a rapid method to characterize the inhibitory effect of amphotericin B(AMB) against Candida albicans by dynamic surface enhanced Raman spectroscopy(D-SERS).Methods D-SERS were applied to analyze the effect of AMB on the Raman spectra of C.albicans by examining the relationship between the peak intensity ratio and the concentration of drug at Raman shift at 656 cm-1 and 729 cm-1 under different interaction time by a sensitive C.albicans and different AMB concentrations.Results The peak intensity ratio was negatively correlated with the concentration of AMB,which could be used to describe the inhibitory effect of AMB on C.albicans in a certain concentration and action time.Conclusion This method could characterize the inhibitory effect of AMB against C.albicans rapidly,which provided a simpler and faster detection method for characterizing more drug-pathogen interactions. |
| keywords:Candida albicans amphotericin B dynamic surface enhanced Raman spectroscopy |
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