| 桃红通脉颗粒质量标准提高研究 |
| 投稿时间：2017-07-20 修订日期：2017-12-22 点此下载全文 |
| 引用本文：金伟华,陈华,张明,于波涛,蒲志强,宋宗辉.桃红通脉颗粒质量标准提高研究[J].药学实践杂志,2018,36(2):162~165,169 |
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| 基金项目:2014年度军队医疗机构制剂标准提高科研专项课题（14ZJZ18-2） |
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| 中文摘要:目的 提高桃红通脉颗粒的质量标准。方法 采用薄层色谱（TLC）法对赤芍、地黄、甘草进行薄层鉴别，以及采用高效液相色谱法对主药桃仁的有效成分苦杏仁苷进行鉴别；采用高效液相色谱法测定芍药苷的含量，色谱柱为Agilent HC-C18柱（250 mm×4.6 mm，5 μm），流动相为乙腈-水（13：87），流速为1.0 ml/min，柱温为30℃，检测波长为230 nm。结果 赤芍、地黄、甘草的TLC图均斑点清晰，阴性无干扰；芍药苷在1.79~57.28 μg/ml范围内浓度与峰面积线性关系良好（r=0.999 9），平均回收率为99.99%，RSD为2.05%（n=9）。结论 提高桃红通脉颗粒质量标准后，鉴别方法重现性更好，优化了芍药苷的含量测定方法，增强了成品质量的可控性。 |
| 中文关键词:桃红通脉颗粒 薄层色谱 高效液相色谱 芍药苷 |
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| Study on improving the quality standard of Taohong Tongmai granule |
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| Abstract:Objective To improve the quality standard of Taohong Tongmai granule. Methods TLC was used to make qualitative identification of Paeoniae Radix Rubra,Rehmanniae Radix,Glycyrrhizae Radix; The effective components of the main walnut kernel were identified by HPLC; and HPLC was used to do quantitative determination of Paeoniflorin,the determination was performed on Agilent HC-C18(250mm×4.6mm,5μm)column with mobile phase consisted of Acetonitrile-water (13:87) at the flow rate of 1.0 ml/min, the column temperature was 30℃ and the detection wavelength was set at 230 nm. Results These TLC spots were fairly clear, and the blank test showed no interference;The paeoniflorin at the range of 1.79-57.28 μg/ml was linear with peak area(r=0.999 9),its average recovery rate was 99.99% and RSD was 2.05%(n=9). Conclusion The quality standard of Taohong Tongmai granule had been improved,identification method was better reproducibility, the determination method of paeoniflorin content had enhanced the controllability of product quality. |
| keywords:Taohong Tongmai granule TLC HPLC Paeoniflorin |
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