| 康咳灵合剂质量标准研究 |
| 投稿时间：2017-01-13 修订日期：2017-04-10 点此下载全文 |
| 引用本文：彭敏,童晓东,杨水英,许彦,姜晓燕.康咳灵合剂质量标准研究[J].药学实践杂志,2017,35(6):539~542 |
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| 基金项目:南通市卫生局青年科研基金（WQ2015065） |
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| 中文摘要:目的 建立康咳灵合剂的质量标准。方法 采用薄层色谱法（TLC）对矮地茶、百部进行定性鉴别；采用高效液相色谱法（HPLC）测定岩白菜素的含量。色谱柱为Lichrospher C18柱（4.6 mm×250 mm，5 μm）；流动相：甲醇-水（20：80）；检测波长：275 nm；柱温：30℃；流速：1 ml/min。结果 TLC法能准确鉴别矮地茶和百部，斑点清晰；岩白菜素在0.064 8~0.648 μg（r=0.9998）范围内线性关系良好，平均回收率100.24%（RSD为1.9%，n=6）。结论 本实验建立的方法简便、专属性高、重复性好，结果准确可靠，可用于康咳灵合剂的质量控制。 |
| 中文关键词:康咳灵合剂 薄层色谱法 高效液相色谱法 质量标准 |
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| Quality standards for Kangkeling mixture |
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| Abstract:Objective To establish quality standards for Kangkeling mixture.Methods Ardisiae Japonicae Herba, Stemonae Radix were identified by TLC. The content of bergenin was determined by HPLC. Lichrospher C18 column(5 μm,4.6 mm×250 mm)was used with methanol-water (20:80) as mobile phase. The flow rate was 1.0 ml/min. Column temperature was 30℃ and the detection wavelength at 275 nm.Results The linear range of bergenin was in the range of 0.064 8-0.648 μg (r=0.999 8). The average recovery was 100.24% (RSD=1.9%, n=6). Conclusion The method is simple, specific and sensitive. It can be used to control the quality of Kangkeling mixture. |
| keywords:Kangkeling mixture TLC HPLC quality standard |
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