| 氟尿嘧啶对结直肠癌RKO细胞SHP2基因表达的影响 |
| 投稿时间：2017-06-19 修订日期：2017-07-10 点此下载全文 |
| 引用本文：王洁,黄岩,张川,马兴元,常文军,陆一鸣.氟尿嘧啶对结直肠癌RKO细胞SHP2基因表达的影响[J].药学实践杂志,2017,35(6):512~515 |
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| 基金项目:国家自然科学基金资助项目（30500093，81274162）；国家科技部"重大新药创制"专项（2009ZX09103-690）；上海市科委生物医药领域科技支撑项目（16431904400）；上海市教委科研创新项目（14ZZ077） |
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| 中文摘要:目的 探究氟尿嘧啶（5-FU）对结直肠癌RKO细胞SHP2基因表达的影响。方法 培养对数生长期的人结直肠癌细胞RKO，5-FU处理48 h后，采用免疫荧光和Western免疫印迹观察SHP2的蛋白表达水平；将能够特异性抑制SHP2表达的siRNA（siSHP2）转染RKO细胞，5-FU处理48 h，CCK8测定细胞吸光度（A）值，流式检测细胞凋亡，观察RKO对5-FU的敏感性变化及5-FU诱导细胞凋亡的影响。结果 结直肠癌细胞RKO在5-FU处理48 h后，其细胞核中SHP2的蛋白表达水平明显增高；siSHP2转染后，RKO细胞对5-FU的敏感性降低，且降低了5-FU诱导的细胞凋亡率。结论 5-FU可能通过影响SHP2的表达，促进RKO细胞的凋亡，发挥抑癌作用。 |
| 中文关键词:氟尿嘧啶 蛋白酪氨酸磷酸酶 结直肠癌 免疫荧光 |
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| Effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO |
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| Abstract:Objective To investigate the effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO.Methods The human colorectal cancer cell line RKO of logarithmic growth phase was cultured 48 h with 5-FU. The expression of SHP2 was observed by immunofluorescence and Western blotting; siSHP2 which specifically inhibits the expression of SHP2 was transfected into RKO cells and cultured 48 h with 5-FU. The cell absorbance (A) values were measured with CCK8 and apoptosis was determined by flow cytometry. The sensitivity of RKO to 5-FU and the effect of 5-FU on apoptosis of RKO cells were observed.Results The expression of nuclear protein of SHP2 was improved remarkably after colorectal cancer cell RKO was cultured 48 h with 5-FU. The sensitivity of RKO cells to 5-FU and the cell apoptosis rate induced by 5-FU were decreased after siSHP2 transfection. Conclusion 5-FU exerts anti-cancer activity possibly due to its promoting the apoptosis of RKO cells by influencing the expression of SHP2. |
| keywords:5-FU SHP2 colorectal cancer cells immunofluorescence |
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