| HPLC法测定冬柏通淋合剂中盐酸小檗碱的含量 |
| 投稿时间：2017-03-02 修订日期：2017-05-31 点此下载全文 |
| 引用本文：侯晓丽,谭朝丹,时扣荣,顾伟鹰,陈伟成.HPLC法测定冬柏通淋合剂中盐酸小檗碱的含量[J].药学实践杂志,2017,35(4):353~354,378 |
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| 基金项目:上海市进一步加快中医药事业发展三年行动计划项目（ZY3-JSFC-2-1011）；上海中医药大学附属第七人民医院"七院新星"人才培养计划（XX2016-10） |
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| 中文摘要:目的 建立HPLC法测定冬柏通淋合剂中盐酸小檗碱的含量。方法 采用 Kromasil C18色谱柱（150 mm×4.6 mm，5 μm）；流动相：乙腈-0.05 mol/L磷酸二氢钠（用磷酸调节pH=3）（28∶72）；流速：1.0 ml/min；柱温：室温（25 ℃）；检测波长：345 nm。结果 盐酸小檗碱在1.00~50.00 μg/ml范围内线性关系良好（r=0.999 6），平均回收率为104.70%，RSD为1.60%（n=5）。结论 该方法准确、灵敏度高、专属性强、重现性好，对提高冬柏通淋合剂的质量控制标准具有参考意义。 |
| 中文关键词:冬柏通淋合剂 盐酸小檗碱 高效液相色谱 |
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| Berberine hydrochloride assay in Dongbai Tonglin Heji by HPLC method |
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| Abstract:Objective To establish a HPLC method for determination of berberine hydrochloride in Dongbai Tonglin Heji. Methods The separation column of Kromasil C18(150 mm×4.6 mm,5 μm) was used. The mobile phase was acetonitrile and 0.05 mol/L NaH2PO4 solution(pH was adjusted to about 3 with phosphoric acid). The flow rate was 1.0 ml/min with the column at room temperature and the detection wavelengths at 345 nm for berberine hydrochloride. Results The linear range of berberine hydrochloride was 1.00~50.00 μg/ml(r=0.999 6). The average recoveries of berberine hydrochloride were 104.70%(RSD 1.60%, n=5). Conclusion This method is accurate, sensitive, selective and reproducible. It provides an alternative method to improve the quality control of Dongbai Tonglin Heji. |
| keywords:Dongbai Tonglin Heji berberine hydrochloride HPLC |
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