| 高效液相色谱法测定鼻炎灵胶囊中黄芩苷含量 |
| 投稿时间：2015-03-13 修订日期：2015-07-12 点此下载全文 |
| 引用本文：郭良君,孙亮,郑巍,陆萍,傅明强,郭建斌.高效液相色谱法测定鼻炎灵胶囊中黄芩苷含量[J].药学实践杂志,2017,35(1):64~66 |
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| 中文摘要:目的 建立高效液相色谱法测定鼻炎灵胶囊中黄芩苷的含量。方法 色谱柱为Waters XBridge C18柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.8%甲酸（25：75），检测波长276 nm，流速1.0 ml/min，柱温30℃，进样量10 μl。结果 黄芩苷在1.25~40 μg/ml范围内（r=0.999 9）呈良好的线性关系，精密度实验中RSD均小于2%，加样回收率在95%~100%之间。结论 该测定方法简便、准确，分离效果好，可用于鼻炎灵胶囊的质量控制。 |
| 中文关键词:高效液相色谱法 鼻炎灵胶囊 黄芩苷 含量测定 |
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| Determination of baicalin in Biyanling capsule by HPLC |
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| Abstract:Objective To develop a HPLC method for determination of baicalin.Methods The separation was carried out on a Waters XBridge C18 column(4.6 mm×250 mm, 5 μm), the mobile phase was composed of acetonitrile and 0.8% formic acid (25:75), the detection wavelength was set at 276 nm, the flow rate was 1.0 ml/min, the column temperature was 30℃ and the injection volume was 10 μl.Results The linearity was obtained over 1.25-40 μg/ml(r=0.999 9) for baicalin. The RSD of precision were less than 2%. The average recovery was between 95% and 100%.Conclusion This HPLC method was simple, accuracy and suitable for the quality control of Biyanling capsule. |
| keywords:HPLC Biyanling capsule baicalin content determination |
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