| 复方汉防己颗粒中野黄芩苷的定性鉴别和含量测定 |
| 投稿时间：2014-12-01 修订日期：2015-03-23 点此下载全文 |
| 引用本文：张淑瑜,徐建江,于燕莉,毕云生,赵文静,刘倩,刘同琪.复方汉防己颗粒中野黄芩苷的定性鉴别和含量测定[J].药学实践杂志,2016,34(3):245~248 |
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| 中文摘要:目的 建立对复方汉防己颗粒中来源于半枝莲的有效成分野黄芩苷的定性鉴别和含量测定方法。方法 采用薄层色谱法对野黄芩苷进行定性鉴别,采用高效液相色谱法进行含量测定。结果 薄层色谱图斑点显色清晰,阴性对照无干扰;含量测定野黄芩苷在22.4~156.8 μg/ml质量浓度范围内与峰面积呈良好线性关系,r=0.9996(n=5),平均加样回收率为97.79%,RSD=0.32%(n=6)。结论 所建立方法专属性强、重现性好,可作为复方汉防己颗粒的质量控制方法。 |
| 中文关键词:复方汉防己颗粒 半枝莲 野黄芩苷 薄层色谱法 高效液相色谱法 |
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| Identification and determination of scutellarin in compound granule tetrandra |
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| Abstract:Objective To develop the methods for identification and determination of scutellarin in compound Granule Tetrandra. Methods A TLC method was used to identify quality for scutellarin, and a HPLC method was used to determine the content of scutellarin. Results The spot was clear in TLC identification without interference of negative control. The sample size of scutellarin had a good linear relationship with peak area r=0.9996(n=5)when ranged from 22.4~156.8 μg/ml, with the average recovery rate 97.79%(RSD=0.32%, n=6). Conclusion The method is simple, with good specificity and reproducibility, which can be used as the quality control for this preparation. |
| keywords:compound granule tetrandra sculellaria barbata scutellarin TLC HPLC |
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