| PVP和PEG表面修饰对有序介孔碳纳米粒分散性及细胞毒性的影响 |
| 投稿时间：2015-10-16 修订日期：2016-01-21 点此下载全文 |
| 引用本文：王琳召,乐李敬,康安锋,佘岚,王欢,马志强,杨峰.PVP和PEG表面修饰对有序介孔碳纳米粒分散性及细胞毒性的影响[J].药学实践杂志,2016,34(2):158~162 |
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| 基金项目:国家自然科学基金(21401214);第二军医大学青年启动基金(2013QN03) |
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| 中文摘要:目的 通过聚乙烯吡咯烷酮(PVP)和培化磷脂酰乙醇胺(DSPE-mPEG2000)对制备的有序介孔碳纳米粒(MCN)进行表面修饰,以改善材料的疏水性质,并考察其对MCN分散性和细胞毒性的影响。方法 采用低浓度水热法合成MCN,并用PVP和DSPE-mPEG2000对其表面修饰,采用透射电镜(TEM)、扫描电镜(SEM)、氮气吸附脱附仪、粒径仪和红外(IR)等表征其性质。考察了表面修饰对MCN分散性的影响,并用CCK-8法考察材料的细胞毒性,用流式细胞术检测材料对细胞氧化应激的影响。结果 制备的MCN粒径分布均一,平均粒径约90 nm,修饰后的粒径略有增大,Zeta电位稍有升高,分散性明显提高,但修饰前后材料对小鼠成纤维细胞(L929)和宫颈癌细胞(HeLa)的毒性没有显著性差异,在相同浓度下,修饰后的MCN能明显减少细胞氧化应激的产生。结论 MCN具有良好的生物相容性,用PVP和DSPE-mPEG2000修饰后的MCN能明显减少细胞氧化应激的发生。 |
| 中文关键词:有序介孔碳纳米粒 聚乙烯吡咯烷酮 培化磷脂酰乙醇胺 表面修饰 细胞毒性 |
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| Effects on the dispersion and cytotoxicity of ordered mesoporous carbon nanoparticles modified with PVP or PEG |
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| Abstract:Objective To improve the hydrophobic properties of the ordered mesoporous carbon nanoparticles(MCN) by PVP or DSPE-mPEG2000 modification, and to compare the effect on the dispersion and cytotoxicity of MCN before and after modification. Methods MCN was synthesized by low concentration hydrothermal method. The surface was modified by PVP or DSPE-mPEG2000. The properties of the materials were characterized by transmission electron microscopy(TEM), scanning electron microscopy(SEM), nitrogen sorption analysis, particle size and infrared spectroscopy(IR). The effect on the dispersion of MCN was investigated. CCK-8 method was used to investigate the cytotoxicity of the materials. Flow cytometry was used to investigate the effect of cell oxidative stress. Results The distribution of synthesized MCN was uniform, with average particle size about 90 nm. The particle size and Zeta potential increased slightly after surface modification, while the dispersibility was significantly improved. No significant difference was observed in the toxicity of L929 and HeLa cells before and after modification. However, the modified MCN showed significant effect of reducing production of cellular reactive oxygen species. Conclusion Ordered mesoporous carbon nanoparticles with surface modified by PVP or DSPE-mPEG2000 provide good biocompatibility and can significantly reduce oxidative stress. |
| keywords:ordered mesoporous carbon nanoparticles PVP DSPE-mPEG2000 surface modification cytotoxicity |
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