| 高效液相色谱法测定气管炎颗粒中黄芩苷的含量 |
| 投稿时间：2014-07-04 修订日期：2014-11-27 点此下载全文 |
| 引用本文：刘彬果,张新萍.高效液相色谱法测定气管炎颗粒中黄芩苷的含量[J].药学实践杂志,2015,33(5):451~452 |
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| 中文摘要:目的 建立高效液相色谱法测定气管炎颗粒中黄芩苷的含量。 方法 采用HPLC法,色谱柱:ZORBAX SB-C18柱(4.6 mm×150 mm,5 μm),以甲醇-0.1%磷酸(47:53)为流动相,流速为1 ml/min,检测波长为280 nm。 结果 黄芩苷在6.63~210 μg/ml范围内呈良好线性关系(r=0.999 8,n=6),平均加样回收率为98.16%,RSD为1.89%。 结论 本法简便、灵敏、准确、重复性好,结果可靠,可有效地控制气管炎颗粒的质量。 |
| 中文关键词:气管炎颗粒 黄芩苷 高效液相色谱法 |
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| Determination of the content of baicalin in Qiguanyan granules by HPLC |
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| Abstract:Objective To establish a method of HPLC for the determination of baicalin in Qiguanyan granules. Methods HPLC was performed on ZORBAX SB-C18(4.6 mm×150 mm,5 μm) column with mobile phase consisted of methanol-0.1% phosphoric acid (47:53) at ambient temperature.The flow rate was 1.0 ml/min.The detection wavelength was 280 nm. Results Baicalin showed a good linearity in the range of 6.63-210 μg/ml(r=0.999 8,n=6),and the average recovery was 98.16% (RSD=1.89%). Conclusion This methods was simple,accurate,sensitive,reproducible and reliable,which was suitable for the content control of Qiguanyan granules. |
| keywords:Qiguanyan granules baicalin HPLC |
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