| 高效液相色谱法测定山楂精降脂片中齐墩果酸和熊果酸含量 |
| 投稿时间：2014-07-09 修订日期：2014-11-07 点此下载全文 |
| 引用本文：林轶男,金慧子,苏娟.高效液相色谱法测定山楂精降脂片中齐墩果酸和熊果酸含量[J].药学实践杂志,2015,33(5):448~450,466 |
| 摘要点击次数: 1725 |
| 全文下载次数: 500 |
|
|
| 中文摘要:目的 建立高效液相色谱法(HPLC)测定山楂精降脂片中齐墩果酸和熊果酸的含量,有效地控制产品质量。 方法 采用Agilent Zorbax SB C18 柱(250 mm×4.6 mm,5 μm),以甲醇-0.06 mol/L乙酸铵(85:15)为流动相,流速为0.8 ml/min;检测波长为210 nm;柱温为25 ℃。 结果 齐墩果酸和熊果酸分别在0.124~2.48 μg(r=0.999 7)和0.498~9.96 μg(r=0.999 8)范围内与其峰面积呈良好线性关系。测得齐墩果酸平均回收率为96.9%,RSD为1.26%;熊果酸平均回收率为100.4%,RSD为2.6%。 结论 山楂精降脂片质量稳定,质控方法简便、准确,结果可靠、重现性好 。 |
| 中文关键词:山楂精降脂片 齐墩果酸 熊果酸 高效液相色谱法 |
| |
| Determination of oleanolic acid and ursolic acid in Shanzhajing Jiangzhi tablet by HPLC |
|
|
| Abstract:Objective To establish the quality standard for Shanzhajing Jiangzhi tablet by HPLC. Methods Samples were analyzed on a Agilent Zorbax SB C18 column(250 mm×4.6 mm,5 μm) with methanol and 0.06 mol/L ammonium acetate(85:15)as mobile phase at the flow rate of 0.8 ml/min. The wavelength and column temperature were set at 210 nm and 25 ℃, respectively. Results The calibration curve showed a good linear relationship within the range of 0.124-2.48 μg for oleanolic acid (r=0.999 7) and 0.498-9.96 μg for ursolic acid (r=0.999 8). The average recovery ratio of the oleanolic acid and ursolic acid were 96.9% (RSD=1.26%) and 100.4% (RSD=2.6%), respectively. Conclusion The method was proved to be good at evaluation effectiveness and practicality. |
| keywords:Shanzhajing Jiangzhi tablet oleanolic acid ursolic acid HPLC |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
| 关闭 |
|
|
|
