| 高效液相色谱法测定新保肾片中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的含量 |
| 投稿时间：2014-05-27 修订日期：2014-07-30 点此下载全文 |
| 引用本文：乔立业,方李,曹燕丽,陆崟,苏华,王曙东.高效液相色谱法测定新保肾片中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的含量[J].药学实践杂志,2015,33(5):438~440 |
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| 基金项目:全军医疗机构制剂标准提高科研专项课题重点项目(13ZJZ13) |
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| 中文摘要:目的 建立新保肾片中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的含量测定方法,为生产质量提供保障。方法 采用高效液相色谱法测定,色谱柱为Lichrospher-C18柱(250 mm ×4.6 mm,5 μm),流动相为甲醇:0.1%磷酸溶液(7030),流速为1.0 ml/min,柱温为35 ℃,检测波长为254 nm。结果 芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚分别在2.30~18.4 μg/ml(r=1.0)、2.930~23.44 μg/ml(r=1.0)、5.00~40.0 μg/ml(r=1.0)、14.870~118.96 μg/ml (r=0.999 8)、7.410~59.28 μg/ml(r=0.999 9)范围内有良好的线性关系,平均回收率分别为100.16%、102.91%、99.76%、100.32%、100.44%,RSD分别为1.58%、1.27%、1.67%、1.33%、1.03%(n=9)。结论 该方法准确易行,便于质量控制。 |
| 中文关键词:芦荟大黄素 大黄酸 大黄素 大黄酚 大黄素甲醚 高效液相色谱法 含量测定 |
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| Determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablets by HPLC |
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| Abstract:Objective To establish method of the determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablet,for the production of quality protection.Methods HPLC was performed on a Lichrospher-C18 column(250 mm×4.6mm,5 μm)with the mobile phase of methanol-0.1% H3PO4 (70:30).The flow rate was 1.0 ml/min,the column temperature was at 35 ℃ and the detection wavelength was 254 nm.Results The linear rang of aloe-emodin,rhein,emodin,chrysophanol and physcion were obtained between 2.30-18.4 μg/ml ( r=1.0),2.930-23.44 μg/ml( r=1.0),5.00-40.0 μg/ml ( r=1.0),14.870-118.96 μg/ml( r= 0.9998 ) and 7.410-59.28 μg/ml(r=0.999 9),the average recoveries were 100.16%,102.91%,99.76%,100.32%,100.44%,RSD were 1.58%,1.27%,1.67%,1.33%,1.03% (n=9).Conclusion The improved method was accurate and feasible which could be used convenient in quality control. |
| keywords:aloe-emodin rhein emodin chrysophanol physcion HPLC content determination |
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