| 海洋拟诺卡菌SCSIO 11492中次生代谢产物的分离及其抗肿瘤活性研究 |
| 投稿时间：2014-12-31 修订日期：2015-04-09 点此下载全文 |
| 引用本文：苏瑞强,李晏,彭坤,李洁,杨全,杨献文.海洋拟诺卡菌SCSIO 11492中次生代谢产物的分离及其抗肿瘤活性研究[J].药学实践杂志,2015,33(5):406~410 |
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| 基金项目:国家自然科学基金(21002110,21372233) |
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| 中文摘要:目的 探索海洋来源的拟诺卡菌(Nocardiopsis sp.) SCSIO 11492的抗肿瘤活性次生代谢产物。 方法 采用硅胶、凝胶、ODS等色谱分离方法对海洋来源的拟诺卡菌SCSIO 11492的发酵粗提物进行分离、纯化,根据次生代谢产物的核磁数据及相关的理化性质确定化合物的结构,最后利用活性测试发现活性成分。 结果 从海洋拟诺卡菌SCSIO 11492中分离纯化得到5个化合物,经鉴定分别为2'-脱氧腺苷(2'-deoxyadenosine,1)、2'-脱氧胸腺嘧啶核苷(2'- deoxythymidine,2)、2'-脱氧尿苷(2'-deoxyuidine,3)、尿嘧啶核苷 (uridine,4)、1-O-棕榈酰基-3-O-β-D-半乳糖基甘油酯(1-O-hexadecanoyl-3-O-β-D-galactopyranosylglycerol,5)。活性测试结果表明化合物5对RAW264.7显示出较好的生长抑制活性(IC50=10.9 μmol/L)。 结论 从海洋拟诺卡菌SCSIO 11492中分离得到5个化合物,均为首次从该属中分离得到。其中化合物5可能是该菌抗肿瘤的活性成分。 |
| 中文关键词:海洋放线菌 拟诺卡菌 次生代谢产物 抗肿瘤 |
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| Sepration and antitumor activities of secondary metabolites from marine actinomycete Nocardiopsis sp. SCSIO 11492 |
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| Abstract:Objective To explore cytotoxic secondary metabolites from a marine actinomycete Nocardiopsis sp. SCSIO 11492. Methods Isolation and purification were carried out by column chromatography over silica gel, Sephadex LH-20, and ODS structures of the isolates were identified mainly by NMR spectroscopic data. And cytotoxic bioassay was performed using MTT method. Results Five compounds were identified as 2'-deoxyadenosine (1), 2'-deoxythymidine (2), 2'-deoxyuidine (3), uridine (4), 1-O-palmitoyl-3-d-galactosyl-sn-glycerol spongilipid (5). Compound 5 exhibited weak cytotoxic activity with IC50 value of 10.9 μmol/L. Conclusion Five compounds were obtained from a marine actinomycete Nocardiopsis sp. SCSIO 11492. All five compounds were reported for the first time from this genus. Compound 5 could be the bioactive compound responsible for the cytotoxic activity of Nocardiopsis sp. SCSIO 11492. |
| keywords:marine actinomycete Nocardiopsis sp. secondary metabolites anti-tumor |
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