| 正交试验法优选葛花异黄酮的提取工艺 |
| 投稿时间：2013-09-29 修订日期：2014-04-15 点此下载全文 |
| 引用本文：王金凤,赵楠,魏颖,杨翠燕,王芳,刘丹.正交试验法优选葛花异黄酮的提取工艺[J].药学实践杂志,2015,33(4):338~340,369 |
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| 基金项目:吉林省科技发展计划项目(20090919,20130101 145JC) |
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| 中文摘要:目的 优选葛花异黄酮的提取工艺。 方法 乙醇回流提取葛花异黄酮,采用紫外分光光度(UV)法和高效液相色谱(HPLC)法测定葛花中总黄酮及鸢尾苷、鸢尾苷元含量。在对乙醇浓度、溶剂用量、提取时间等进行单因素考察基础上,选用L9(34)正交设计试验优选提取工艺。 结果 葛花异黄酮的最佳提取工艺为第1次加入12倍量70%乙醇提取90 min,第2次加入10倍量70%乙醇提取60 min。 结论 优选的葛花异黄酮提取工艺合理可行,能为实际生产提供参考。 |
| 中文关键词:葛花 鸢尾苷 鸢尾苷元 提取工艺 正交试验 |
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| Optimized extraction technology of flos Puerariae lobata isoflavone using orthogonal test |
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| Abstract:Objective To optimize the extraction technology of flos Puerariae lobata isoflavone. Methods The flos Puerariae lobata isoflavone was distilled by ethanol circumfluence. Total flavonoids, tectoridin and tectorigenin extracted from Puerariae using the UV and HPLC spectromertry methods were taken as evaluation indexes. Extraction technology was optimized with L9(34) orthogonal test on the base of single observation of ethanol concentration, solvent dosage and distilling time. Results The best extraction technology of flos Puerariae lobata isoflavone was: to add 12 times the amount of 70% ethanol for 90 minutes for the first time, and 10 times the amount of 70% ethanol for 60 minutes for the second time. Conclusion The optimized extraction process of flos Puerariae lobata isoflavone is reasonable and feasible, and it can offer reference to actual production. |
| keywords:flos Puerariae lobata tectoridin tectorigenin extract technology orthogonal test |
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