| 荔枝核皂苷和荔枝核黄酮对HepG2细胞胰岛素抵抗作用研究 |
| 投稿时间：2015-04-22 修订日期：2015-06-06 点此下载全文 |
| 引用本文：肖志军,郭洁文,徐峰.荔枝核皂苷和荔枝核黄酮对HepG2细胞胰岛素抵抗作用研究[J].药学实践杂志,2015,33(4):316~318,350 |
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| 基金项目:上海市卫生局中医药科研基金 (No. 2012Y010A) |
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| 中文摘要:目的 探讨荔枝核皂苷、荔枝核黄酮对胰岛素抵抗HepG2细胞糖代谢的影响。 方法 采用高浓度胰岛素培养基诱导HepG2细胞形成胰岛素抵抗模型,葡萄糖氧化酶法测定对照组、荔枝核皂苷组、荔枝核黄酮组、罗格列酮组细胞培养上清液中的葡萄糖含量,观察荔枝核皂苷、荔枝核黄酮对HepG2细胞胰岛素抵抗的作用。 结果 HepG2细胞在10-6mol/L的胰岛素中作用24 h,细胞对胰岛素的抵抗作用最为明显。以此条件构建胰岛素抵抗细胞模型,对该模型给予荔枝核皂苷、荔枝核黄酮干预后,细胞培养上清液中葡葡糖含量未能显著降低。 结论 本研究提示荔枝核皂苷、荔枝核黄酮不能有效改善胰岛素抵抗。 |
| 中文关键词:荔枝核皂苷 荔枝核黄酮 胰岛素抵抗 HepG2细胞 |
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| Effect of litchi saponin and litchi flavones on insulin resistance in HepG2 cells |
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| Abstract:Objective To study the effect of litchi saponin and flavones on glycometabolism in insulin resistance model of HepG2 cells. Methods Using high insulin in HepG2 cells to establish a cell model of insulin resistance. Cell model was treated by litchi saponin, litchi flavones and rosiglitazone, respectively. Glucose concentration of cell culture supernatant was detected by glucose oxidase method. Results In the concentration of 10-6 mol/L insulin for 24 hours, HepG2 reached the highest level of resistant to insulin, which means a successful insulin resistance cell model was established. However, no significant decrease in glucose concentration of cell culture supernatant was observed with litchi saponin and flavones. Conclusion This study suggests that litchi saponin and flavones have no effect to improve insulin resistance. |
| keywords:litchi saponin litchi flavones insulin resistance HepG2 cells |
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