| HPLC-DAD法同时测定肝八味胶囊中4种成分的含量 |
| 投稿时间：2014-02-18 修订日期：2014-12-12 点此下载全文 |
| 引用本文：吴丽红,储忠英,刘惠军.HPLC-DAD法同时测定肝八味胶囊中4种成分的含量[J].药学实践杂志,2015,33(3):250~252 |
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| 中文摘要:目的 用高效液相色谱(HPLC)法同时测定肝八味胶囊中4种成分的含量。 方法 采用HPLC法, 色谱柱为C18柱(250 mm×4.6 mm,5 μm), 以乙腈为流动相A, 以水为流动相B, 梯度洗脱; 检测波长为240 nm; 流速1.0 ml/min。 结果 丹酚酸B对照品在15.026~100.17 μg/ml范围内呈良好线性关系,平均加样回收率为96.6%(n=6),RSD=1.8%(n=6);芍药苷对照品在14.335~95.564 μg/ml范围内呈良好线性关系,平均加样回收率为97.0%(n=6),RSD=1.7%(n=6);虎杖苷对照品在8.235~54.90 μg/ml范围内呈良好线性关系,平均加样回收率为97.2%(n=6),RSD=1.8%(n=6);大黄素对照品在1.582 5~10.55 μg/ml范围内呈良好线性关系,平均加样回收率为98.1%(n=6),RSD=1.7%(n=6)。 结论 此法简单准确、重现性好、专属性强、阴性对照无干扰,适用于肝八味胶囊的质量控制。 |
| 中文关键词:肝八味胶囊 高效液相色谱法 丹酚酸B 芍药苷 虎杖苷 大黄素 |
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| Simultaneous determination of four ingredients in Gan Bawei capsule by HPLC-DAD |
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| Abstract:Objective To establish an HPLC-DAD method for simultaneous determination of four ingredients in Gan Bawei capsule. Methods HPLC method was adopted with C18 column(250 mm ×4.6 mm, 5 μm), Acetonitrile as mobile phase A and water as mobile phase B in gradient elution mode. The detection wavelength was 240 nm and the flow rate was 1.0 ml/min. Results Salvianolic acid B reference substance was excellently linear in the range of 15.026~100.17 μg/ml, the average recovery was 96.6%(n=6)and RSD was 1.8%(n=6). Paeoniflorin reference substance was excellently linear in the range of 14.335~95.564 μg/ml, the average recovery was 97.0%(n=6)and RSD was 1.7%(n=6).Polydatin reference substance was excellently linear in the range of 8.235-54.90 μg/ml, the average recovery was 97.2%(n=6)and RSD was 1.8%(n=6).Emodin reference substance was excellently linear in the range of 1.582 5-10.55 μg/ml, the average recovery was 98.1%(n=6)and RSD was 1.7%(n=6). Conclusion The method was easy, accurate, reproducible, specific and without interference in negative control, which could be used for quality control of Gan Bawei capsule. |
| keywords:Gan Bawei capsule HPLC salvianolic acid B paeoniflorin polydatin emodin |
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