| 用高效液相色谱法测定银翘散配方颗粒中连翘酯苷A的含量 |
| 投稿时间：2013-08-10 修订日期：2014-02-20 点此下载全文 |
| 引用本文：王婵,韦刃,黄晓玉,林广,黄志权,周永强.用高效液相色谱法测定银翘散配方颗粒中连翘酯苷A的含量[J].药学实践杂志,2015,33(2):156~158 |
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| 基金项目:南宁市本级科学研究与技术开发计划项目(20141060,20133163);广西创新计划专项经费项目(2014CXJHA02) |
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| 中文摘要:目的 建立了银翘散配方颗粒中连翘酯苷A的含量测定方法。 方法 采用高效液相色谱法,色谱柱为Welch Ultimate XB-C18(250 mm×4.6 mm,5 μm),以甲醇-0.2%冰醋酸溶液为流动相进行梯度洗脱,流速为1.0 ml/min,检测波长为330 nm,柱温20 ℃。 结果 连翘酯苷A进样量在0.080 3~3.211 μg范围内线性关系良好(r=1.000)。加样回收率为103.78%,RSD为0.82%。 结论 该方法简便、准确,专一性和重复性较好,可用于银翘散配方颗粒的质量控制。 |
| 中文关键词:高效液相色谱法 银翘散配方颗粒 连翘酯苷A |
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| Content determination of Forsythoside A in Yinqiaosan granules by HPLC |
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| Abstract:Objective To establish a method for determination of the content of Forsythoside A in Yinqiaosan granules. Methods HPLC method was adopted to carry on the determination. Welch Ultimate XB-C18 column (250 mm×4.6 mm,5 μm) was used with the mobile phase of methanol-0.2% Glacial acetic acid solution by gradient elution. The flow rate was 1.0 ml/min, the detection wavelength was 330 nm, and the column temperature was 20 ℃. Results Forsythoside A showed a good linear relationship at the range from 0.080 3~3.211 μg (r=1.000).The average recovery rates were 103.78%, and RSD=0.82%. Conclusion The method was convenient and accurate. Meanwhile, it also has good specificity and reproducibility. It can be used effectively for the quality control of Yinqiaosan granules. |
| keywords:HPLC Yinqiaosan granules Forsythoside A |
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