| 正交试验优选元宝草中总黄酮的提取工艺 |
| 投稿时间：2013-10-17 修订日期：2014-03-17 点此下载全文 |
| 引用本文：龚颖珍,郑承剑,秦路平.正交试验优选元宝草中总黄酮的提取工艺[J].药学实践杂志,2015,33(2):134~137 |
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| 基金项目:上海市科委中药现代化专项 (2011DZ1970502) |
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| 中文摘要:目的 优选元宝草中总黄酮的提取工艺。 方法 在单因素试验的基础上,考察乙醇浓度、料液比、提取温度等因素对元宝草中总黄酮提取率的影响,并采用L9(3)4正交试验优选最佳提取条件。 结果 影响元宝草中总黄酮提取率的因素顺序为乙醇浓度>料液比>提取次数>提取温度。元宝草最佳提取工艺条件为70%乙醇,提取温度60 ℃,乙醇用量20倍,提取次数3次。该条件下元宝草中总黄酮平均得率为2.38%。 结论 本实验设计合理、测定方法可行、实验结果可靠,为大批量从元宝草中提取总黄酮提供了理论依据。 |
| 中文关键词:元宝草 总黄酮 提取工艺 正交试验 |
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| Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments |
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| Abstract:Objective To optimize the extraction technology of total falvonoids from Hypericum sampsonii. Methods On the basis of single factor test, solid-liquid ratio, ethanol concentration, extraction time, extraction temperature and extraction times on the extraction rate were studied by taking the content of total falvonoids as indexes. Orthogonal test was conducted to establish the extraction technology of total falvonoids from Hypericum sampsonii. Results The influence degree of factors on the extraction rate of total falvonoids was in the order of ethanol concentration, solid-liquid ratio, extraction times, and temperature.The optimum extraction condition was confirmed as follows: ethanol concentration of 70%, extraction temperature of 60 ℃, and extraction times of 3×1.5 h, solid to liquid ratio of 120. Under this condition,the average extraction rate of total falvonoids reached 2.38%. Conclusion The test was reasonable in design and credible in findings, and was easily operated, which could provide scientific basis for the batch extraction of total flavonoids from Hypericum sampsonii. |
| keywords:Hypericum sampsonii total flavonoids extracting technology orthogonal test |
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