| 高效液相色谱法测定盐酸氟西汀的含量 |
| 投稿时间：2013-05-22 修订日期：2014-04-07 点此下载全文 |
| 引用本文：吴荷琴,陈朝霞.高效液相色谱法测定盐酸氟西汀的含量[J].药学实践杂志,2015,33(1):58~59,82 |
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| 中文摘要:目的 建立测定盐酸氟西汀含量的高效液相色谱法。方法 选用Agilent Eclipse XDB-C8柱(4.6 mm×250 mm,5 μm);流动相:四氢呋喃-甲醇-三乙胺缓冲液(取三乙胺10 ml,置1 000 ml量筒中,加水980 ml,用磷酸调节pH值至6.0)(30:10:60);流速为1.0 ml/min;检测波长:227 nm;进样量:10 μl;柱温:25℃。结果 方法的线性范围为55.17~165.51 μg/ml(r=0.999 9);最低检测限:0.15 μg/ml;回收率在99.9%~100.0%之间,重复性RSD为0.1%(n=6)。结论 方法简便灵敏, 结果准确可靠,可用于盐酸氟西汀的质量控制。 |
| 中文关键词:盐酸氟西汀 含量测定 高效液相色谱法 |
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| Determination of fluoxetine hydrochloride by HPLC |
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| Abstract:Objective To establish an HPLC method for assay determination of fluoxetine hydrochloride. Methods Agilent Eclipse XDB-C8 (4.6 mm × 250 mm,5 μm) was used,mobile phase was tetrahydrofuran-methanol-triethylamine buffer (to 10 ml of triethylamine in a 1 000 ml flask,added 980 ml of water,pH was adjust to 6.0 by phosphoric acid) (30:10:60),flow rate was 1.0 ml/min,detection wavelength was 227 nm,injection volume was 10 μl,column temperature was 25℃. Results Linearity range was 55.17-165.51 μg/ml (r=0.999 9),minimum detection limit was 0.15 μg/ml,accuracy was between 99.9%-100.0%,repeatability RSD was 0.1% (n=6). Conclusion The method was accurate and reliable,which could be applied for quality control of fluoxetine hydrochloride. |
| keywords:fluoxetine hydrochloride assay HPLC |
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